Adaptive reduction of male gamete number in the selfing plant Arabidopsis thaliana.

Autor:	Tsuchimatsu T; Department of Evolutionary Biology and Environmental Studies & Zurich-Basel Plant Science Center, University of Zurich, 8057, Zurich, Switzerland.; Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008, Zurich, Switzerland.; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, A-1030, Vienna, Austria.; Department of Biology, Chiba University, Chiba, 263-8522, Japan.; Department of Biological Sciences, University of Tokyo, Tokyo, 113-0033, Japan., Kakui H; Department of Evolutionary Biology and Environmental Studies & Zurich-Basel Plant Science Center, University of Zurich, 8057, Zurich, Switzerland.; Kihara Institute for Biological Research, Yokohama City University, Yokohama, 244-0813, Japan.; Graduate School of Science and Technology, Niigata University, Niigata, 950-2181, Japan., Yamazaki M; Department of Evolutionary Biology and Environmental Studies & Zurich-Basel Plant Science Center, University of Zurich, 8057, Zurich, Switzerland., Marona C; Institute for Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany., Tsutsui H; Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008, Zurich, Switzerland.; Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan.; JST ERATO Higashiyama Live-Holonics Project, Nagoya University, Nagoya, 464-8602, Japan., Hedhly A; Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008, Zurich, Switzerland., Meng D; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, A-1030, Vienna, Austria.; Molecular and Computational Biology, University of Southern California, Los Angeles, CA, 90089-0371, USA., Sato Y; Department of Genomics and Evolutionary Biology, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan., Städler T; Institute of Integrative Biology, ETH Zurich, 8092, Zurich, Switzerland., Grossniklaus U; Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008, Zurich, Switzerland., Kanaoka MM; Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan., Lenhard M; Institute for Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany., Nordborg M; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, A-1030, Vienna, Austria., Shimizu KK; Department of Evolutionary Biology and Environmental Studies & Zurich-Basel Plant Science Center, University of Zurich, 8057, Zurich, Switzerland. kentaro.shimizu@ieu.uzh.ch.; Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, 8008, Zurich, Switzerland. kentaro.shimizu@ieu.uzh.ch.; Kihara Institute for Biological Research, Yokohama City University, Yokohama, 244-0813, Japan. kentaro.shimizu@ieu.uzh.ch.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2020 Jun 08; Vol. 11 (1), pp. 2885. Date of Electronic Publication: 2020 Jun 08.
DOI:	10.1038/s41467-020-16679-7
Abstrakt:	The number of male gametes is critical for reproductive success and varies between and within species. The evolutionary reduction of the number of pollen grains encompassing the male gametes is widespread in selfing plants. Here, we employ genome-wide association study (GWAS) to identify underlying loci and to assess the molecular signatures of selection on pollen number-associated loci in the predominantly selfing plant Arabidopsis thaliana. Regions of strong association with pollen number are enriched for signatures of selection, indicating polygenic selection. We isolate the gene REDUCED POLLEN NUMBER1 (RDP1) at the locus with the strongest association. We validate its effect using a quantitative complementation test with CRISPR/Cas9-generated null mutants in nonstandard wild accessions. In contrast to pleiotropic null mutants, only pollen numbers are significantly affected by natural allelic variants. These data support theoretical predictions that reduced investment in male gametes is advantageous in predominantly selfing species.
Databáze:	MEDLINE
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