| Autor: |
Andrzejczak OA; Department of Agroecology, Aarhus University, Forsøgsvej 1, DK, 4200, Slagelse, Denmark., Sørensen CK; Department of Agroecology, Aarhus University, Forsøgsvej 1, DK, 4200, Slagelse, Denmark., Wang WQ; Department of Biochemistry & Molecular Biology and VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Campusvej 55, DK, 5230, Odense M, Denmark.; Key Laboratory of Plant Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, 100093, Beijing, China., Kovalchuk S; Department of Biochemistry & Molecular Biology and VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Campusvej 55, DK, 5230, Odense M, Denmark.; Laboratory of Bioinformatic methods for Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia., Hagensen CE; Department of Biochemistry & Molecular Biology and VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Campusvej 55, DK, 5230, Odense M, Denmark., Jensen ON; Department of Biochemistry & Molecular Biology and VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Campusvej 55, DK, 5230, Odense M, Denmark., Carciofi M; Department of Agroecology, Aarhus University, Forsøgsvej 1, DK, 4200, Slagelse, Denmark.; Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, DK, 4200, Slagelse, Denmark., Hovmøller MS; Department of Agroecology, Aarhus University, Forsøgsvej 1, DK, 4200, Slagelse, Denmark., Rogowska-Wrzesinska A; Department of Biochemistry & Molecular Biology and VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Campusvej 55, DK, 5230, Odense M, Denmark., Møller IM; Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, DK, 4200, Slagelse, Denmark., Hebelstrup KH; Department of Agroecology, Aarhus University, Forsøgsvej 1, DK, 4200, Slagelse, Denmark. kim.hebelstrup@mbg.au.dk.; Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, DK, 4200, Slagelse, Denmark. kim.hebelstrup@mbg.au.dk. |
| Abstrakt: |
Nonhost resistance, a resistance of plant species against all nonadapted pathogens, is considered the most durable and efficient immune system in plants. To increase our understanding of the response of barley plants to infection by powdery mildew, Blumeria graminis f. sp. tritici, we used quantitative proteomic analysis (LC-MS/MS). We compared the response of two genotypes of barley cultivar Golden Promise, wild type (WT) and plants with overexpression of phytoglobin (previously hemoglobin) class 1 (HO), which has previously been shown to significantly weaken nonhost resistance. A total of 8804 proteins were identified and quantified, out of which the abundance of 1044 proteins changed significantly in at least one of the four comparisons ('i' stands for 'inoculated')- HO/WT and HOi/WTi (giving genotype differences), and WTi/WT and HOi/HO (giving treatment differences). Among these differentially abundant proteins (DAP) were proteins related to structural organization, disease/defense, metabolism, transporters, signal transduction and protein synthesis. We demonstrate that quantitative changes in the proteome can explain physiological changes observed during the infection process such as progression of the mildew infection in HO plants that was correlated with changes in proteins taking part in papillae formation and preinvasion resistance. Overexpression of phytoglobins led to modification in signal transduction prominently by dramatically reducing the number of kinases induced, but also in the turnover of other signaling molecules such as phytohormones, polyamines and Ca 2+ . Thus, quantitative proteomics broaden our understanding of the role NO and phytoglobins play in barley during nonhost resistance against powdery mildew. |
