Chronic rhinosinusitis: assessment of changes in nociceptive neurons.

Autor: Bogaert S; Department of Head and Skin, Upper Airway Research Laboratory (URL), Ghent University Hospital, Ghent University, Ghent, Belgium.; Department of Otorhinolaryngology, Head and Neck Surgery, St. Elisabeth-Hospital, Ruhr University Bochum, Bochum, Germany., Van Crombruggen K; Department of Head and Skin, Upper Airway Research Laboratory (URL), Ghent University Hospital, Ghent University, Ghent, Belgium., Holtappels G; Department of Head and Skin, Upper Airway Research Laboratory (URL), Ghent University Hospital, Ghent University, Ghent, Belgium., De Ruyck N; Department of Head and Skin, Upper Airway Research Laboratory (URL), Ghent University Hospital, Ghent University, Ghent, Belgium., Suchonos N; Department of Otorhinolaryngology, Head and Neck Surgery, St. Elisabeth-Hospital, Ruhr University Bochum, Bochum, Germany., Park JJ; Department of Otorhinolaryngology, Head and Neck Surgery, Witten/Herdecke University, Hagen, Germany., Bachert C; Department of Head and Skin, Upper Airway Research Laboratory (URL), Ghent University Hospital, Ghent University, Ghent, Belgium.; Division of ENT Diseases, CLINTEC, Karolinska Institute, Stockholm, Sweden.
Jazyk: angličtina
Zdroj: International forum of allergy & rhinology [Int Forum Allergy Rhinol] 2020 Oct; Vol. 10 (10), pp. 1165-1172. Date of Electronic Publication: 2020 Jun 07.
DOI: 10.1002/alr.22603
Abstrakt: Background: Pain is a major symptom of chronic rhinosinusitis (CRS). It is mainly associated with CRS without nasal polyps (CRSsNP) and has a major impact in the decision to move on to surgery. Patients with CRS with nasal polyps (CRSwNP) are characterized by trigeminal hypoesthesia and suffer from less pain. The aim of this study was to investigate whether CRS induces alterations in the peripheral nociceptive neurons, mainly focusing on quantitative changes.
Methods: Sinus mucosa and inferior turbinate (IT) samples were obtained from patients with CRS, and IT tissue of healthy patients served as controls. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed for neuronal markers including CNTNAP2, FAM19A1, GFRA2, NEFH, NTRK1, PLXNC1, RET, SCN10A, SCN11A, TRPV1, and PGP 9.5; enzyme-linked immunosorbent assay (ELISA) was performed for KCNK18, SCN10A, MRGPRD, and MAP2. For PGP 9.5, immunohistochemistry was additionally used to analyze tissue slides.
Results: We included 35 patients with CRSsNP, 47 patients with CRSwNP, and 18 control patients. No differences in expression of the neuronal markers were observed between CRSsNP, CRSwNP, and controls. SCN10A was the only marker exclusively expressed on nociceptive neurons in sinus tissue. No histological difference in nerve fibers was observed between sinus mucosa of both phenotypes.
Conclusion: Our results indicate that the nociceptive nerve density in CRSwNP is not lower than in CRSsNP, as was assumed previously. The nociceptive neurons in sinonasal mucosa cannot be classified into subtypes due to the lack of specificity of the respective marker genes. Our findings question the generally accepted claim that nasal polyp tissue does not contain any nerves.
(© 2020 ARS-AAOA, LLC.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje