Measuring thiamine status in dried blood spots.

Autor: Huang Y; Department of Wine and Food Science, School of Agriculture, Food and Wine, the University of Adelaide, Adelaide, SA 5065 Australia. Electronic address: yichao.huang@jnu.edu.cn., Gibson RA; Department of Wine and Food Science, School of Agriculture, Food and Wine, the University of Adelaide, Adelaide, SA 5065 Australia; Women and Kids theme, South Australia Health and Medical Research Institute (SAHMRI), Adelaide, SA 5006, Australia., Green TJ; Women and Kids theme, South Australia Health and Medical Research Institute (SAHMRI), Adelaide, SA 5006, Australia; Discipline of Paediatrics, University of Adelaide, Adelaide, SA 5000 Australia.
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2020 Oct; Vol. 509, pp. 52-59. Date of Electronic Publication: 2020 Jun 04.
DOI: 10.1016/j.cca.2020.06.011
Abstrakt: Background: A reliable and robust method with minimum sample collection requirement for thiamine assay is needed in clinical and research settings.
Methods: A simple and robust assay for three vitamers (thiamine, Th; thiamine monophosphate, TMP; and thiamine diphosphate, TDP) using a 6.35-mm dried blood spot (DBS) disc was developed, validated and applied.
Results: We were able to quantify accurately thiamine status covering all major vitamers Th, TMP and TDP with acceptable recovery (90%-114%), limit of quantification (TDP: 3.0 nM, TMP and Th: 1.5 nM), linearity (TDP: LOQ 400 nM, TMP and Th: LOQ 50 nM, all R 2  > 0.99), imprecision (coefficient variation < 4.3% for TDP, <10.0% for TMP and < 12.6% for Th) and stability at -20 °C for up to 42 days. By recruiting 20 healthy participants, we cross compared finger capillary DBS with venous whole blood and venous blood pre-spotted on filter papers. The results demonstrated minimum bias between methods. A preliminary dosing study showed the method had excellent sensitivity after a single dose of supplemental thiamine.
Conclusions: We have developed and clinically validated a simple, robust, accurate and sensitive assay for the analysis of thiamine status in DBS, suitable for large-scale population studies.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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