Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian Spermatogenesis.

Autor:	Shami AN; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA., Zheng X; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA., Munyoki SK; Department of Obstetrics, Gynecology, and Reproductive Sciences, Integrative Systems Biology Graduate Program, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Ma Q; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA., Manske GL; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA., Green CD; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA., Sukhwani M; Department of Obstetrics, Gynecology, and Reproductive Sciences, Integrative Systems Biology Graduate Program, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Orwig KE; Department of Obstetrics, Gynecology, and Reproductive Sciences, Integrative Systems Biology Graduate Program, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Electronic address: korwig@mwri.magee.edu., Li JZ; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA; Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA. Electronic address: junzli@med.umich.edu., Hammoud SS; Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA; Department of Urology, University of Michigan, Ann Arbor, MI, USA. Electronic address: hammou@med.umich.edu.
Jazyk:	angličtina
Zdroj:	Developmental cell [Dev Cell] 2020 Aug 24; Vol. 54 (4), pp. 529-547.e12. Date of Electronic Publication: 2020 Jun 05.
DOI:	10.1016/j.devcel.2020.05.010
Abstrakt:	Spermatogenesis is a highly regulated process that produces sperm to transmit genetic information to the next generation. Although extensively studied in mice, our current understanding of primate spermatogenesis is limited to populations defined by state-specific markers from rodent data. As between-species differences have been reported in the duration and differentiation hierarchy of this process, it remains unclear how molecular markers and cell states are conserved or have diverged from mice to man. To address this challenge, we employ single-cell RNA sequencing to identify transcriptional signatures of major germ and somatic cell types of the testes in human, macaque, and mice. This approach reveals similarities and differences in expression throughout spermatogenesis, including the stem/progenitor pool of spermatogonia, markers of differentiation, potential regulators of meiosis, RNA turnover during spermatid differentiation, and germ cell-soma communication. These datasets provide a rich foundation for future targeted mechanistic studies of primate germ cell development and in vitro gametogenesis. Competing Interests: Declaration of Interests The authors declare no competing interests. (Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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