| Autor: |
Lawson PA; Department of Microbiology and Plant Biology, University of Oklahoma, Norman, USA., Patel NB; Department of Microbiology and Plant Biology, University of Oklahoma, Norman, USA., Mohammed A; University of Baghdad School of Veterinary Medicine, Baghdad, Iraq.; Department of Pathology, Microbiology and Immunology, University of South Carolina, Columbia, SC, USA., Moore ERB; Culture Collection, Department of Clinical Bacteriology, University of Göteborg, S-41346 Göteborg, Sweden., Lo AS; Beale Air Force Base, CA, USA., Sardi A; Department of Surgical Oncology, Institute for Cancer Care at Mercy Medical Center, Baltimore, MD, USA., Davis JM; Department of Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, SC, USA., Doyle DA; Department of Microbiology and Plant Biology, University of Oklahoma, Norman, USA., Hui Y; Department of Pathology, Microbiology and Immunology, University of South Carolina, Columbia, SC, USA., Testerman T; Department of Pathology, Microbiology and Immunology, University of South Carolina, Columbia, SC, USA. |
| Abstrakt: |
A Gram-stain-negative, microaerophilic, non-motile, rod-shaped bacterium strain designated PMP191F T , was isolated from a human peritoneal tumour. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism formed a lineage within the family Chitinophagaceae that was distinct from members of the genus Pseudoflavitalea (95.1-95.2 % sequence similarity) and Pseudobacter ginsenosidimutans (94.4 % sequence similarity). The average nucleotide identity values between strain PMP191F T and Pseudoflavitalea rhizosphaerae T16R-265 T and Pseudobacter ginsenosidimutans Gsoil 221 T was 68.9 and 62.3% respectively. The only respiratory quinone of strain PMP191F T was MK-7 and the major fatty acids were iso-C 15 : 0 , iso-C 15 : 1 G and summed feature 3 (C 16:1 ω7 c and/or C 16:1 ω6 c ). The polar lipids consisted of phosphatidylethanolamine and some unidentified amino and glycolipids. The G+C content of strain PMP191F T calculated from the genome sequence was 43.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, strain PMP191F T represents a novel species and genus for which the name Parapseudoflavitalea muciniphila gen. nov., sp. nov. is proposed. The type strain is PMP191F T (=DSM 104999 T =ATCC BAA-2857 T = CCUG 72691 T ). The phylogenetic analyses also revealed that Pseudobacter ginsenosidimutans shared over 98 % sequence similarly to members of the genus Pseudoflavitalea . However, the average nucleotide identity value between Pseudoflavitalea rhizosphaerae T16R-265 T , the type species of the genus and Pseudobacter ginsenosidimutans Gsoil 221 T was 86.8 %. Therefore, we also propose that Pseudobacter ginsenosidimutans be reclassified as Pseudoflavitalea ginsenosidimutans comb. nov. |
