Robotic microscopy for everyone: the OpenFlexure microscope.

Autor:	Collins JT; Centre for Photonics and Photonic Materials, Department of Physics, University of Bath, UK., Knapper J; Centre for Photonics and Photonic Materials, Department of Physics, University of Bath, UK., Stirling J; Centre for Photonics and Photonic Materials, Department of Physics, University of Bath, UK., Mduda J; Ifakara Health Institute, Ifakara, Tanzania., Mkindi C; Ifakara Health Institute, Ifakara, Tanzania., Mayagaya V; Ifakara Health Institute, Ifakara, Tanzania., Mwakajinga GA; STICLab, Dar Es Salaam, Tanzania., Nyakyi PT; STICLab, Dar Es Salaam, Tanzania., Sanga VL; STICLab, Dar Es Salaam, Tanzania., Carbery D; Department of Chemistry, University of Bath, UK., White L; Department of Chemistry, University of Bath, UK., Dale S; Centre for Nanoscience and Nanotechnology, Department of Physics, University of Bath, UK., Jieh Lim Z; Centre for Nanoscience and Nanotechnology, Department of Physics, University of Bath, UK., Baumberg JJ; Nanophotonics Centre, Cavendish Laboratory, University of Cambridge, UK., Cicuta P; Cavendish Laboratory, University of Cambridge, UK., McDermott S; Cavendish Laboratory, University of Cambridge, UK., Vodenicharski B; Cavendish Laboratory, University of Cambridge, UK., Bowman R; Centre for Photonics and Photonic Materials, Department of Physics, University of Bath, UK.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2020 Apr 08; Vol. 11 (5), pp. 2447-2460. Date of Electronic Publication: 2020 Apr 08 (Print Publication: 2020).
DOI:	10.1364/BOE.385729
Abstrakt:	Optical microscopes are an essential tool for both the detection of disease in clinics, and for scientific analysis. However, in much of the world access to high-performance microscopy is limited by both the upfront cost and maintenance cost of the equipment. Here we present an open-source, 3D-printed, and fully-automated laboratory microscope, with motorised sample positioning and focus control. The microscope is highly customisable, with a number of options readily available including trans- and epi- illumination, polarisation contrast imaging, and epi-florescence imaging. The OpenFlexure microscope has been designed to enable low-volume manufacturing and maintenance by local personnel, vastly increasing accessibility. We have produced over 100 microscopes in Tanzania and Kenya for educational, scientific, and clinical applications, demonstrating that local manufacturing can be a viable alternative to international supply chains that can often be costly, slow, and unreliable. Competing Interests: The authors declare no conflicts of interest. (Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.)
Databáze:	MEDLINE
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