Cultivation dependent formation of siderophores by Gordonia rubripertincta CWB2.

Autor: Schwabe R; Institute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany; Institute of Biosciences, Biology and Ecology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany. Electronic address: ringoschwabe007@gmail.com., Senges CHR; Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, Universitätsstraße 150, 44801 Bochum, Germany., Bandow JE; Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, Universitätsstraße 150, 44801 Bochum, Germany., Heine T; Institute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany., Lehmann H; Institute of Informatic, TU Bergakademie Freiberg, Bernhard-von-Cotta Straße 2, 09599 Freiberg, Germany., Wiche O; Institute of Biosciences, Biology and Ecology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany., Schlömann M; Institute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany., Levicán G; Laboratorio de Microbiología Básica y Aplicada, Facultad de Química y Biología, Universidad de Santiago, Chile., Tischler D; Institute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany; Microbial Biotechnology, Faculty of Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany. Electronic address: dirk.tischler@rub.de.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2020 Sep; Vol. 238, pp. 126481. Date of Electronic Publication: 2020 May 26.
DOI: 10.1016/j.micres.2020.126481
Abstrakt: Herein we demonstrate cultivation-dependent siderophore production by the actinomycete Gordonia rubripertincta CWB2. The strain produces mostly citrate, but also desferrioxamine E (DFOE) and new hydroxamate-type siderophores. The production of hydroxamate-like siderophores is influenced by cultivation conditions, for example available carbon sources or presence of metals, such as the rare earth erbium or the heavy metal lead. By cultivation with succinate and extraction with an adsorbing resin (XAD) we purified the G. rubripertincta CWB2 siderophores (yield up to 178 mg L -1 ). The respective workflow comprises genome mining, cultivation, and overproduction strategies, a rapid screening procedure, as well as traditional structure enrichment and structure elucidation methods. This combination of methods allows the discovery of new natural products with metal complexation capacity, also for lanthanides of commercial value. G. rubripertincta CWB2 carries a desferrioxamine-like biosynthetic gene cluster. Its transcription was proven by a transcriptomic approach comparing expression levels of the selected gene cluster during cultivation in iron-depleted and repleted media. Further investigation of the siderophores of this desferrioxamine producing Actinobacterium could lead to new structures.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2020 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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