Insights into the unique carboxylation reactions in the metabolism of propylene and acetone.

Autor:	Mus F; Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, U.S.A., Wu HH; Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, U.S.A.; Department of Chemistry, Washington State University, WA 99164., Alleman AB; Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, U.S.A., Shisler KA; Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, U.S.A., Zadvornyy OA; Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, U.S.A., Bothner B; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, U.S.A., Dubois JL; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, U.S.A., Peters JW; Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, U.S.A.
Jazyk:	angličtina
Zdroj:	The Biochemical journal [Biochem J] 2020 Jun 12; Vol. 477 (11), pp. 2027-2038.
DOI:	10.1042/BCJ20200174
Abstrakt:	Alkenes and ketones are two classes of ubiquitous, toxic organic compounds in natural environments produced in several biological and anthropogenic processes. In spite of their toxicity, these compounds are utilized as primary carbon and energy sources or are generated as intermediate metabolites in the metabolism of other compounds by many diverse bacteria. The aerobic metabolism of some of the smallest and most volatile of these compounds (propylene, acetone, isopropanol) involves novel carboxylation reactions resulting in a common product acetoacetate. Propylene is metabolized in a four-step pathway involving five enzymes where the penultimate step is a carboxylation reaction catalyzed by a unique disulfide oxidoreductase that couples reductive cleavage of a thioether linkage with carboxylation to produce acetoacetate. The carboxylation of isopropanol begins with conversion to acetone via an alcohol dehydrogenase. Acetone is converted to acetoacetate in a single step by an acetone carboxylase which couples the hydrolysis of MgATP to the activation of both acetone and bicarbonate, generating highly reactive intermediates that are condensed into acetoacetate at a Mn2+ containing the active site. Acetoacetate is then utilized in central metabolism where it is readily converted to acetyl-coenzyme A and subsequently converted into biomass or utilized in energy metabolism via the tricarboxylic acid cycle. This review summarizes recent structural and biochemical findings that have contributed significant insights into the mechanism of these two unique carboxylating enzymes. (© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
Databáze:	MEDLINE
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