A Spore-Forming Probiotic Supplement Improves the Intestinal Immune Response and Protects the Intestinal Health During Recurrent Clostridioides difficile Colonization in Mice.

Autor: Ramezani Kapourchali F; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA., Glueck B; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA., Han Y; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA., Shapiro D; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA., Fulmer CG; Robert J. Tomsich Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, Ohio, USA., Cresci GAM; Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA.; Department of Pediatric Gastroenterology, Cleveland Clinic, Cleveland, Ohio, USA.; Center for Human Nutrition, Cleveland Clinic, Cleveland, Ohio, USA.
Jazyk: angličtina
Zdroj: JPEN. Journal of parenteral and enteral nutrition [JPEN J Parenter Enteral Nutr] 2020 Nov; Vol. 44 (8), pp. 1428-1438. Date of Electronic Publication: 2020 Jun 04.
DOI: 10.1002/jpen.1851
Abstrakt: Background: Around 15%-30% of patients develop recurrent Clostridioides difficile infection (CDI) as conventional therapies disrupt protective gut microbiota. We tested if supplementation with a spore-forming probiotic would protect intestinal health in a mouse model of recurrent CD colonization.
Methods: Methods: Female CF-1 mice were exposed to CD spores (4-log 10 colony-forming units/10 μL) and then randomly assigned to receive either saline (CD-S) or probiotic (CD-PRO). Control mice received only saline (control). Following confirmation of initial CD colonization, mice were treated with vancomycin (10 days). After 5 days, mice recolonized with CD were treated again with vancomycin (10 days) and euthanized 5 days later. Fecal samples were collected at select time points for bacterial analysis. Following euthanasia, blood samples, cecum contents, and the intestine were collected for analysis.
Results: Probiotic supplementation mitigated the antibiotic-induced changes in cecum weight (P < .001). Probiotic-supplemented mice had increased messenger RNA expression of several immune parameters, accompanied by lower serum iron levels compared with CD-S mice (P < .05). Lower expressions of TNF α and calprotectin (P ≤ .05) were observed in CD-PRO mice compared with CD-S. The probiotics also supported the expression of intestinal tight junction proteins, which were diminished in the proximal colon of CD-S mice (P < .05).
Conclusion: Mice supplemented with targeted spore-forming probiotics exhibited improved immune responses and nutrition immunity properties, which were linked with less inflammation and enhanced intestinal barrier proteins during recurrent CD colonization.
(© 2020 American Society for Parenteral and Enteral Nutrition.)
Databáze: MEDLINE
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