Exosome-templated nanoplasmonics for multiparametric molecular profiling.

Autor:	Wu X; Institute for Health Innovation and Technology, National University of Singapore, Singapore 117599, Singapore., Zhao H; Institute for Health Innovation and Technology, National University of Singapore, Singapore 117599, Singapore., Natalia A; Institute for Health Innovation and Technology, National University of Singapore, Singapore 117599, Singapore.; Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore 117583, Singapore., Lim CZJ; Institute for Health Innovation and Technology, National University of Singapore, Singapore 117599, Singapore.; Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore 117583, Singapore., Ho NRY; Institute for Health Innovation and Technology, National University of Singapore, Singapore 117599, Singapore.; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore 138673, Singapore., Ong CJ; Division of Surgical Oncology, National Cancer Centre, Singapore 169610, Singapore., Teo MCC; Division of Surgical Oncology, National Cancer Centre, Singapore 169610, Singapore., So JBY; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore.; Division of Surgical Oncology, National University Cancer Institute, Singapore 169610, Singapore., Shao H; Institute for Health Innovation and Technology, National University of Singapore, Singapore 117599, Singapore.; Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore 117583, Singapore.; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore 138673, Singapore.; Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2020 May 06; Vol. 6 (19), pp. eaba2556. Date of Electronic Publication: 2020 May 06 (Print Publication: 2020).
DOI:	10.1126/sciadv.aba2556
Abstrakt:	Exosomes are nanoscale vesicles distinguished by characteristic biophysical and biomolecular features; current analytical approaches, however, remain univariate. Here, we develop a dedicated platform for multiparametric exosome analysis-through simultaneous biophysical and biomolecular evaluation of the same vesicles-directly in clinical biofluids. Termed templated plasmonics for exosomes, the technology leverages in situ growth of gold nanoshells on vesicles to achieve multiselectivity. For biophysical selectivity, the nanoshell formation is templated by and tuned to distinguish exosome dimensions. For biomolecular selectivity, the nanoshell plasmonics locally quenches fluorescent probes only if they are target-bound on the same vesicle. The technology thus achieves multiplexed analysis of diverse exosomal biomarkers (e.g., proteins and microRNAs) but remains unresponsive to nonvesicle biomarkers. When implemented on a microfluidic, smartphone-based sensor, the platform is rapid, sensitive, and wash-free. It not only distinguished biomarker organizational states in native clinical samples but also showed that the exosomal subpopulation could more accurately differentiate patient prognosis. (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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