The majority of the matrix protein TapA is dispensable for Bacillus subtilis colony biofilm architecture.

Autor: Earl C; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Arnaouteli S; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Bamford NC; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Porter M; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Sukhodub T; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., MacPhee CE; James Clerk Maxwell Building, School of Physics, University of Edinburgh, Edinburgh, UK., Stanley-Wall NR; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK.
Jazyk: angličtina
Zdroj: Molecular microbiology [Mol Microbiol] 2020 Dec; Vol. 114 (6), pp. 920-933. Date of Electronic Publication: 2020 Jun 21.
DOI: 10.1111/mmi.14559
Abstrakt: Biofilm formation is a co-operative behaviour, where microbial cells become embedded in an extracellular matrix. This biomolecular matrix helps manifest the beneficial or detrimental outcome mediated by the collective of cells. Bacillus subtilis is an important bacterium for understanding the principles of biofilm formation. The protein components of the B. subtilis matrix include the secreted proteins BslA, which forms a hydrophobic coat over the biofilm, and TasA, which forms protease-resistant fibres needed for structuring. TapA is a secreted protein also needed for biofilm formation and helps in vivo TasA-fibre formation but is dispensable for in vitro TasA-fibre assembly. We show that TapA is subjected to proteolytic cleavage in the colony biofilm and that only the first 57 amino acids of the 253-amino acid protein are required for colony biofilm architecture. Through the construction of a strain which lacks all eight extracellular proteases, we show that proteolytic cleavage by these enzymes is not a prerequisite for TapA function. It remains unknown why TapA is synthesised at 253 amino acids when the first 57 are sufficient for colony biofilm structuring; the findings do not exclude the core conserved region of TapA having a second role beyond structuring the B. subtilis colony biofilm.
(© 2020 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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