The rK39 Antigen from an Iranian Strain of Leishmania infantum : Detection of Anti- Leishmania Antibodies in Humans and Dogs.

Autor: Hosseini Farash BR; Department of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.; Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Mohebali M; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.; Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran., Kazemi B; Department of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Hajjaran H; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Fata A; Department of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Raoofian R; Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran., Akhoundi B; Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Mojarrad M; Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Mastroeni P; Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom., Sharifi-Yazdi MK; Center for Research of Zoonoses of Iran, Tehran University of Medical Sciences, Tehran, Iran., Tanipour MH; Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Jazyk: angličtina
Zdroj: Iranian journal of parasitology [Iran J Parasitol] 2020 Jan-Mar; Vol. 15 (1), pp. 48-56.
Abstrakt: Background: Visceral leishmaniasis (VL) is the most severe form of leishmaniasis in Iran with high mortality rates in the case of inaccurate diagnosis and treatment. This study aimed to prepare and evaluate a new rk39 recombinant antigen from an Iranian strain of Leishmania infantum for diagnosis of VL in humans and dogs.
Methods: rK39-based enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for the detection of anti L. infantum antibodies. We screened 84 human sera and 87 dog sera from clinical cases in the endemic area of Meshkin-Shahr, Iran along with 176 sera from healthy controls (collected from 86 humans and 90 dogs) during 2013-2016.
Results: Using the rK39 ELISA, a sensitivity of 85.7% (95% CI, 95-99%) and a specificity of 86.0% (95% CI, 95%-99%) were detected in human sera at a 1:800 (cut-off) titer when DAT-confirmed cases were compared with healthy controls; a sensitivity of 96.6% (95% CI, 95%-99%) and specificity of 94.4% (95% CI, 95%-99%) were found at a 1:80 (cut-off) titer compared with DAT. Kappa analysis indicated agreement between the rK39 ELISA and DAT (0.718) when using human sera at a 1:800 (cut-off) titer as well as (0.910) at a 1:80 (cut-off) titer when using dog sera ( P <0.05).
Conclusion: New rk39 recombinant antigen from an Iranian strain of Leishmania infantum seems to be used for diagnosis of VL in humans and dogs. Further extended field studies are recommended.
Competing Interests: Conflict of interests The authors declare that there is no conflict of interests.
(Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences.)
Databáze: MEDLINE
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