| Autor: |
Radke JR; Research Section, Edward Hines, Jr. VA Hospital, Hines, IL 60141, USA.; Department of Microbiology and Immunology, Loyola University of Chicago-Stritch School of Medicine, Maywood, IL 60153, USA.; Infectious Diseases and Immunology Research Institute, Loyola University Chicago-Stritch School of Medicine, Maywood, IL 60153, USA.; Division of Infectious Diseases, Loyola University Chicago-Stritch School of Medicine, Maywood, IL 60153, USA.; Research Section, Boise VA Hospital, Boise, ID 83702, USA.; Idaho Veterans Research and Education Foundation, Boise, ID 83702, USA., Covert HJ; Research Section, Boise VA Hospital, Boise, ID 83702, USA.; Idaho Veterans Research and Education Foundation, Boise, ID 83702, USA., Bauer F; Research Section, Boise VA Hospital, Boise, ID 83702, USA.; Idaho Veterans Research and Education Foundation, Boise, ID 83702, USA., Ananthanarayanan V; Department of Pathology, Loyola University Chicago-Stritch School of Medicine, Maywood, IL 60153, USA., Cook JL; Research Section, Edward Hines, Jr. VA Hospital, Hines, IL 60141, USA.; Department of Microbiology and Immunology, Loyola University of Chicago-Stritch School of Medicine, Maywood, IL 60153, USA.; Infectious Diseases and Immunology Research Institute, Loyola University Chicago-Stritch School of Medicine, Maywood, IL 60153, USA.; Division of Infectious Diseases, Loyola University Chicago-Stritch School of Medicine, Maywood, IL 60153, USA. |
| Abstrakt: |
Adenovirus (Ad) infections are usually mild and self-limited, with minimal inflammatory responses. During worldwide outbreaks, Ad14p1, an emerging Ad14 variant, has caused severe pulmonary disease, including acute respiratory distress syndrome (ARDS). This increased pathogenicity of Ad14p1 is not completely understood. In initial studies, we observed that infection of Syrian hamsters with Ad14p1 can cause a patchy bronchopneumonia, with an increased intensity of inflammation, compared to wild type Ad14 infection. The current study compared the dynamics of the immunopathogenesis of Ad14 and Ad14p1 infection of hamster lungs through the first two weeks after infection. Little difference was seen in infection-induced inflammation at day 1. Beginning at day 3, Ad14p1-infected hamsters showed marked inflammation that continued through to day 7. The inflammation began to resolve by day 10 but was still detectable at day 14. In contrast, Ad14-infected hamsters showed little inflammation during the 14-day period of observation. Inflammatory cell type analysis revealed that, at day 1, hamsters infected with either virus had predominantly neutrophil infiltration that began to resolve by day 3. However, at day 5, Ad14p1-infected hamsters had a second wave of neutrophil infiltration that was accompanied by edema which persisted to a variable extent through to day 10. These differences were not explained by an increased Ad14p1 replication rate, compared with Ad14 in vitro, but there was prolonged persistence of Ad14p1 in hamster lungs. There were differences in lung tissue cytokine and chemokine responses to Ad14p1 vs. Ad14 infection that might account for the increased leukocyte infiltrates in Ad14p1-infected hamsters. This animal model characterization provides the basis for future translational studies of the viral genetic mechanisms that control the increased immunopathogenesis of the emergent, Ad14p1 strain. |
