Characterization of mechanisms involved in tolerance and accumulation of Cd in Biscutella auriculata L.

Autor: Peco JD; Escuela Técnica Superior de Ingenieros Agrónomos, Universidad de Castilla-La Mancha (UCLM). Ronda de Calatrava, 7, 13071, Ciudad Real, Spain; Instituto de Geología Aplicada, Universidad de Castilla-La Mancha (UCLM). Plaza de Manuel Meca, 1, 13400, Almadén, Ciudad Real, Spain., Campos JA; Escuela Técnica Superior de Ingenieros Agrónomos, Universidad de Castilla-La Mancha (UCLM). Ronda de Calatrava, 7, 13071, Ciudad Real, Spain; Instituto de Geología Aplicada, Universidad de Castilla-La Mancha (UCLM). Plaza de Manuel Meca, 1, 13400, Almadén, Ciudad Real, Spain., Romero-Puertas MC; Department of Biochemistry Cellular and Molecular Biology of Plants, Estación Experimental Del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008, Granada, Spain., Olmedilla A; Department of Biochemistry Cellular and Molecular Biology of Plants, Estación Experimental Del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008, Granada, Spain., Higueras P; Instituto de Geología Aplicada, Universidad de Castilla-La Mancha (UCLM). Plaza de Manuel Meca, 1, 13400, Almadén, Ciudad Real, Spain., Sandalio LM; Department of Biochemistry Cellular and Molecular Biology of Plants, Estación Experimental Del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008, Granada, Spain. Electronic address: luisamaria.sandalio@eez.csic.es.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2020 Sep 15; Vol. 201, pp. 110784. Date of Electronic Publication: 2020 May 30.
DOI: 10.1016/j.ecoenv.2020.110784
Abstrakt: Biscutella auriculata L. is one of the rare species that is able to grow in a very contaminated mining area in Villamayor de Calatrava (Ciudad Real, Spain). In an effort to understand the mechanisms involved in the tolerance of this plant to high metal concentrations, we grew B. auriculata in the presence of 125 μM Cd(NO 3 ) 2 for 15 days and analysed different parameters associated with plant growth, nitric oxide and reactive oxygen species metabolism, metal uptake and translocation, photosynthesis rate and biothiol (glutathione and phytochelatins) content. Treatment with Cd led to growth inhibition in both the leaves and the roots, as well as a reduction of photosynthetic parameters, transpiration and stomatal conductance. The metal was mainly accumulated in the roots and in the vascular tissue, although most Cd was detected in areas surrounding their epidermal cells, while in the leaves the metal accumulated mainly in spongy mesophyll, stomata and trichrome. Based on the Cd bioaccumulation (5.93) and translocation (0.15) factors, this species denoted enrichment of the metal in the roots and its low translocation to the upper tissues. Biothiol analysis showed a Cd-dependent increase of reduced glutathione (GSH) as well as the phytochelatins (PC2 and PC3) in both roots and leaves. Cd-promoted oxidative damage occurred mainly in the leaves due to disturbances in enzymatic and nonenzymatic antioxidants, while the roots did not show significant damage as a result of induction of antioxidant defences. It can be concluded that B. auriculata is a new Cd-tolerant plant with an ability to activate efficient metal-sequestering mechanisms in the root surface and leaves and to induce PCs, as well as antioxidative defences in roots.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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