Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana.

Autor: Masuda HP; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, SP, Brazil; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, SP, Brazil., Nakabashi M; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, SP, Brazil., Morgante PG; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, SP, Brazil; Universidade Estadual Paulista (UNESP), Campus de Registro São Paulo, SP, Brazil., Kajihara D; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, SP, Brazil; Laboratório de Biologia Vascular, Instituto do Coração (InCor) da Faculdade de Medicina, Universidade de São Paulo, SP, Brazil., de Setta N; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, SP, Brazil; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, SP, Brazil., Menck CFM; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil., Van Sluys MA; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, SP, Brazil. Electronic address: mavsluys@usp.br.
Jazyk: angličtina
Zdroj: Gene [Gene] 2020 Sep 05; Vol. 754, pp. 144818. Date of Electronic Publication: 2020 May 30.
DOI: 10.1016/j.gene.2020.144818
Abstrakt: Plants are continuously exposed to agents that can generate DNA lesions. Nucleotide Excision Repair (NER) is one of the repair pathways employed by plants to protect their genome, including from sunlight. The Xeroderma Pigmentosum type B (XPB) protein is a DNA helicase shown to be involved in NER and is also an essential subunitof the Transcription Factor IIH (TFIIH) complex. XPB was found to be a single copy gene in eukaryotes, but found as a tandem duplication in the plant Arabidopsis thaliana, AtXPB1 and AtXPB2. We aimed to investigate whether the XPB in tandem duplication was common within members of the Brassicaceae. We analyzed genomic DNA of species from different tribes of the family and the results indicate that the tandem duplication occurred in Camelineae tribe ancestor, of which A. thaliana belongs, at approximately 8 million years ago. Further experiments were devised to study possible functional roles for the A. thaliana AtXPB paralogs. A non-coincident expression profile of the paralogs was observed in various plant organs, developmental and cell cycle stages. AtXPB2 expression was observed in proliferating cells and clustered with the transcription of other components of the TFIIH such as p44, p52 and XPD/UVH6 along the cell cycle. AtXPB1 gene transcription, on the other hand, was enhanced specifically after UV-B irradiation in leaf trichomes. Altogether, our results reported herein suggest a functional specialization for the AtXPB paralogs: while the AtXPB2 paralog may have a role in cell proliferation and repair as XPB of other eukaryotes, the AtXPB1 paralog is most likely implicated in repair functions in highly specialized A. thaliana cells.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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