A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids.

Autor: Deynoux M; EA 7501 GICC, CNRS ERL 7001 LNOx, Université de Tours, Tours, France., Sunter N; EA 7501 GICC, CNRS ERL 7001 LNOx, Université de Tours, Tours, France., Ducrocq E; EA 7501 GICC, CNRS ERL 7001 LNOx, Université de Tours, Tours, France., Dakik H; EA 7501 GICC, CNRS ERL 7001 LNOx, Université de Tours, Tours, France., Guibon R; Anatomie et cytologie pathologique, CHRU de Tours, Tours, France.; INSERM UMR1069, Nutrition, Croissance et Cancer, Université de Tours, Tours, France., Burlaud-Gaillard J; Plateforme IBiSA de Microscopie Electronique, Université et CHRU de Tours, Tours, France.; INSERM U1259 MAVIVH, Université et CHRU de Tours, Tours, France., Brisson L; INSERM UMR1069, Nutrition, Croissance et Cancer, Université de Tours, Tours, France., Rouleux-Bonnin F; EA 7501 GICC, CNRS ERL 7001 LNOx, Université de Tours, Tours, France., le Nail LR; Service de Chirurgie orthopédique, CHU Tours, Tours, France., Hérault O; EA 7501 GICC, CNRS ERL 7001 LNOx, Université de Tours, Tours, France.; Service d'hématologie biologique, CHRU de Tours, Tours, France., Domenech J; EA 7501 GICC, CNRS ERL 7001 LNOx, Université de Tours, Tours, France.; Service d'hématologie biologique, CHRU de Tours, Tours, France., Roingeard P; Plateforme IBiSA de Microscopie Electronique, Université et CHRU de Tours, Tours, France.; INSERM U1259 MAVIVH, Université et CHRU de Tours, Tours, France., Fromont G; Anatomie et cytologie pathologique, CHRU de Tours, Tours, France.; INSERM UMR1069, Nutrition, Croissance et Cancer, Université de Tours, Tours, France., Mazurier F; EA 7501 GICC, CNRS ERL 7001 LNOx, Université de Tours, Tours, France.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Jun 02; Vol. 15 (6), pp. e0225485. Date of Electronic Publication: 2020 Jun 02 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0225485
Abstrakt: Mesenchymal stem cells (MSC)-spheroid models favor maintenance of stemness, ex vivo expansion and transplantation efficacy. Spheroids may also be considered as useful surrogate models of the hematopoietic niche. However, accessibility to primary cells, from bone marrow (BM) or adipose tissues, may limit their experimental use and the lack of consistency in methods to form spheroids may affect data interpretation. In this study, we aimed to create a simple model by examining the ability of cell lines, from human (HS-27a and HS-5) and murine (MS-5) BM origins, to form spheroids, compared to primary human MSCs (hMSCs). Our protocol efficiently allowed the spheroid formation from all cell types within 24 hours. Whilst hMSC-spheroids began to shrink after 24 hours, the size of spheroids from cell lines remained constant during three weeks. The difference was partially explained by the balance between proliferation and cell death, which could be triggered by hypoxia and induced oxidative stress. Our results demonstrate that, like hMSCs, MSC cell lines make reproductible spheroids that are easily handled. Thus, this model could help in understanding mechanisms involved in MSC functions and may provide a simple model by which to study cell interactions in the BM niche.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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