CRISPR/Cas9 genome editing shows the important role of AZC_2928 gene in nitrogen-fixing bacteria of plants.

Autor: Wang X; College of Science, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China., Lv S; College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China., Liu T; College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China., Wei J; College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China., Qu S; College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China., Lu Y; College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China., Zhang J; College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China., Oo S; Department of Biology, East Carolina University, Greenville, NC, 27858, USA.; Elizabeth City State University, Elizabeth City, NC, 27909, USA., Zhang B; Department of Biology, East Carolina University, Greenville, NC, 27858, USA., Pan X; Department of Biology, East Carolina University, Greenville, NC, 27858, USA. panx@ecu.edu., Liu H; College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China. bioche@nwsuaf.edu.cn.
Jazyk: angličtina
Zdroj: Functional & integrative genomics [Funct Integr Genomics] 2020 Sep; Vol. 20 (5), pp. 657-668. Date of Electronic Publication: 2020 Jun 01.
DOI: 10.1007/s10142-020-00739-8
Abstrakt: AZC_2928 gene (GenBank accession no. BAF88926.1) of Azorhizobium caulinodans ORS571 has sequence homology to 2,3-aminomutases. However, its function is unknown. In this study, we are for the first time to knock out the gene completely in A. caulinodans ORS571 using the current advanced genome editing tool, CRISPR/Cas9. Our results show that the editing efficiency is 34% and AZC_2928 plays an extremely important role in regulating the formation of chemotaxis and biofilm. CRISPR/Cas9 knockout of AZC_2928 (△AZC_2928) significantly enhanced chemotaxis and biofilm formation. Both chemotaxis and biofilm formation play an important role in nitrogen-fixing bacteria and their interaction with their host plants. Interestingly, AZC_2928 did not affect the motility of A. caulinodans ORS571 and the nodulation formation in their natural host plant, Sesbania rostrata. Due to rhizobia needing to form bacteroids for symbiotic nitrogen fixation in mature nodules, AZC_2928 might have a direct influence on nitrogen fixation efficiency rather than the number of nodulations.
Databáze: MEDLINE
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