Integrating whole-genome sequencing within the National Antimicrobial Resistance Surveillance Program in the Philippines.

Autor:	Argimón S; Centre for Genomic Pathogen Surveillance, Wellcome Genome Campus, Hinxton, UK., Masim MAL; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Gayeta JM; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Lagrada ML; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Macaranas PKV; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Cohen V; Centre for Genomic Pathogen Surveillance, Wellcome Genome Campus, Hinxton, UK., Limas MT; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Espiritu HO; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Palarca JC; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Chilam J; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Jamoralin MC Jr; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Villamin AS; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Borlasa JB; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Olorosa AM; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Hernandez LFT; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Boehme KD; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Jeffrey B; Centre for Genomic Pathogen Surveillance, Wellcome Genome Campus, Hinxton, UK., Abudahab K; Centre for Genomic Pathogen Surveillance, Wellcome Genome Campus, Hinxton, UK., Hufano CM; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines.; St. Luke's Medical Center-Global City, Taguig, Metro Manila, The Philippines., Sia SB; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines., Stelling J; Brigham and Women's Hospital, Boston, MA, USA., Holden MTG; University of St Andrews School of Medicine, St Andrews, Scotland, UK., Aanensen DM; Centre for Genomic Pathogen Surveillance, Wellcome Genome Campus, Hinxton, UK. david.aanensen@bdi.ox.ac.uk.; Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, UK. david.aanensen@bdi.ox.ac.uk., Carlos CC; Antimicrobial Resistance Surveillance Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, The Philippines. ccarlosphl@yahoo.com.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2020 Jun 01; Vol. 11 (1), pp. 2719. Date of Electronic Publication: 2020 Jun 01.
DOI:	10.1038/s41467-020-16322-5
Abstrakt:	National networks of laboratory-based surveillance of antimicrobial resistance (AMR) monitor resistance trends and disseminate these data to AMR stakeholders. Whole-genome sequencing (WGS) can support surveillance by pinpointing resistance mechanisms and uncovering transmission patterns. However, genomic surveillance is rare in low- and middle-income countries. Here, we implement WGS within the established Antimicrobial Resistance Surveillance Program of the Philippines via a binational collaboration. In parallel, we characterize bacterial populations of key bug-drug combinations via a retrospective sequencing survey. By linking the resistance phenotypes to genomic data, we reveal the interplay of genetic lineages (strains), AMR mechanisms, and AMR vehicles underlying the expansion of specific resistance phenotypes that coincide with the growing carbapenem resistance rates observed since 2010. Our results enhance our understanding of the drivers of carbapenem resistance in the Philippines, while also serving as the genetic background to contextualize ongoing local prospective surveillance.
Databáze:	MEDLINE
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