Bufadienolide glycosides and bufadienolides from the whole plants of Helleborus lividus, and their cytotoxic activity.

Autor: Iguchi T; Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan., Yokosuka A; Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan. Electronic address: yokosuka@toyaku.ac.jp., Tamura N; Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan., Takano S; Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan., Mimaki Y; Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
Jazyk: angličtina
Zdroj: Phytochemistry [Phytochemistry] 2020 Aug; Vol. 176, pp. 112415. Date of Electronic Publication: 2020 May 29.
DOI: 10.1016/j.phytochem.2020.112415
Abstrakt: Cytotoxicity-guided fractionation of the MeOH extract of Helleborus lividus Aiton ex Curtis (Ranunculaceae) resulted in the isolation of five undescribed bufadienolide glycosides and two undescribed bufadienolides, along with three known compounds. Their structures were determined by detailed spectroscopic analysis and hydrolysis studies. The isolated compounds showed cytotoxicity against HL-60 human leukemia cells and A549 human lung adenocarcinoma cells, with IC 50 values ranging from 2.20 ± 0.01 nM to 0.77 ± 0.01 μM. The undescribed compound 3β-[(O-β-d-glucopyranosyl-(1 → 4)-α-l-rhamnopyranosyl)oxy]-14β,16β-dihydroxy-5β-bufa-20,22-dienolide induced apoptosis in HL-60 cells via a mitochondria-dependent apoptotic pathway. The average IC 50 values of bufadienolide monorhamnosides for HL-60 and A549 cells were 10-20 times lower than those for Na + /K + ATPase, implying that they induce tumor cell death via a mechanism of action other than Na + /K + ATPase inhibition.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest associated with this manuscript.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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