Identification of Pathogenic Leptospira Species in the Urogenital Tract of Water Buffaloes ( Bubalus bubalis ) From the Amazon River Delta Region, Brazil.

Autor:	Guedes IB; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Oliveira de Souza G; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Fernandes de Paula Castro J; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., de Souza Filho AF; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Cavalini MB; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Taniwaki SA; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Maia ALP; Médico Veterinário, Auditor Fiscal Agropecuário, Agência de Defesa e Inspeção Agropecuária Do Estado Do Amapá, Santana, Brazil., Pereira IC; Secretaria de Estado da Saúde Do Amapá, Macapá, Brazil., Heinemann MB; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2020 May 14; Vol. 7, pp. 269. Date of Electronic Publication: 2020 May 14 (Print Publication: 2020).
DOI:	10.3389/fvets.2020.00269
Abstrakt:	In the current context of deforestation and fire in the Amazon, buffaloes could be a cost-effective and sustainable alternative for cattle production in the region, as they can convert low-quality foods and be raised in floodplain areas. However, little is known about the reproductive diseases that affect these animals; thus, the purpose of this study was to perform the molecular characterization of Leptospira spp. in the urogenital tract of water buffaloes ( Bubalus bubalis ) raised in the Amazon River Delta region in Brazil. Samples were collected from 114 kidneys, 204 ovaries, and 160 uterine swabs of slaughtered buffaloes in the Macapá microregion of Amapá State (Brazil) and were subjected to PCR to detect bacterial DNA. Positive amplicons were sequenced to identify Leptospira species. Among the total samples, 11/473 were PCR positive (2.3%), including 10 kidney samples and one uterine swab sample. DNA sequencing identified two pathogenic species from the kidney samples: L. interrogans , accounting for 60.0% (6/10) of these samples, and L. borgpetersenii , accounting for 20.0% (2/10), while 20.0% (2/10) were identified only at the genus level. The bacterium in the uterine swab sample was identified as L. interrogans with genetic proximity to strains belonging to the serovar Hardjo. This is the first report of leptospires species identified in buffaloes from the Amazon River Delta region and revealed that these animals may be carriers of different pathogenic Leptospira species, similar to bovines, including showing genital colonization. (Copyright © 2020 Guedes, Oliveira de Souza, Fernandes de Paula Castro, de Souza Filho, Cavalini, Taniwaki, Maia, Pereira and Heinemann.)
Databáze:	MEDLINE
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