Spatiotemporal Modulation of Flavonoid Metabolism in Blueberries.

Autor:	Günther CS; The New Zealand Institute for Plant & Food Research Ltd., Auckland, New Zealand., Dare AP; The New Zealand Institute for Plant & Food Research Ltd., Auckland, New Zealand., McGhie TK; The New Zealand Institute for Plant & Food Research Ltd., Palmerston North, New Zealand., Deng C; The New Zealand Institute for Plant & Food Research Ltd., Auckland, New Zealand., Lafferty DJ; The New Zealand Institute for Plant & Food Research Ltd., Palmerston North, New Zealand.; School of Biological Sciences, The University of Auckland, Auckland, New Zealand., Plunkett BJ; The New Zealand Institute for Plant & Food Research Ltd., Auckland, New Zealand., Grierson ERP; The New Zealand Institute for Plant & Food Research Ltd., Palmerston North, New Zealand., Turner JL; The New Zealand Institute for Plant & Food Research Ltd., Brooklyn, New Zealand., Jaakola L; Climate Laboratory Holt, Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.; Norwegian Institute of Bioeconomy Research, Norwegian Institute of Bioeconomy Research, Ås, Norway., Albert NW; The New Zealand Institute for Plant & Food Research Ltd., Palmerston North, New Zealand., Espley RV; The New Zealand Institute for Plant & Food Research Ltd., Auckland, New Zealand.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2020 May 13; Vol. 11, pp. 545. Date of Electronic Publication: 2020 May 13 (Print Publication: 2020).
DOI:	10.3389/fpls.2020.00545
Abstrakt:	Blueberries are distinguished by their purple-blue fruit color, which develops during ripening and is derived from a characteristic composition of flavonoid-derived anthocyanin pigments. The production of anthocyanins is confined to fruit skin, leaving the colorless fruit flesh devoid of these compounds. By linking accumulation patterns of phenolic metabolites with gene transcription in Northern Highbush ( Vaccinium corymbosum ) and Rabbiteye ( Vaccinium virgatum) blueberry, we investigated factors limiting anthocyanin production in berry flesh. We find that flavonoid production was generally lower in fruit flesh compared with skin and concentrations further declined during maturation. A common set of structural genes was identified across both species, indicating that tissue-specific flavonoid biosynthesis was dependent on co-expression of multiple pathway genes and limited by the phenylpropanoid pathway in combination with CHS , F3H , and ANS as potential pathway bottlenecks. While metabolite concentrations were comparable between the blueberry genotypes when fully ripe, the anthocyanin composition was distinct and depended on the degree of hydroxylation/methoxylation of the anthocyanidin moiety in combination with genotype-specific glycosylation patterns. Co-correlation analysis of phenolic metabolites with pathway structural genes revealed characteristic isoforms of O -methyltransferases and UDP-glucose:flavonoid-3- O -glycosyltransferase that were likely to modulate anthocyanin composition. Finally, we identified candidate transcriptional regulators that were co-expressed with structural genes, including the activators MYBA , MYBPA1 , and bHLH2 together with the repressor MYBC2 , which suggested an interdependent role in anthocyanin regulation. (Copyright © 2020 Günther, Dare, McGhie, Deng, Lafferty, Plunkett, Grierson, Turner, Jaakola, Albert and Espley.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu