| Autor: |
Jitsatja A; Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand., Ramphan S; Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand., Promma P; Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand., Kuadkitkan A; Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand., Wikan N; Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand., Uiprasertkul M; Department of Pathology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand., Phatihattakorn C; Department of Obstetrics and Gynaecology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand., Smith DR; Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand. duncan_r_smith@hotmail.com. |
| Abstrakt: |
In this study, we compared the characteristics of two strains of Zika virus (ZIKV) isolated in Thailand, one isolated from a febrile patient and one isolated from tissues of a fetus medically terminated due to congenital Zika syndrome (CZS). Replication profiles showed that the isolate from the fetal tissues replicated significantly more slowly than the fever-associated isolate in human lung A549 cells during the first 24 hours postinfection but showed a similar growth profile over longer-term infection. A much smaller difference was observed in Aedes albopictus C6/36 cells. In a quasispecies analysis, a high proportion (approximately 20%) of nonfunctional genomes was identified, caused by an adenine insertion in the prM gene. This insertion was found to be present in two Thai fever strains and as such may represent a common feature of Thai endemic ZIKV. Comparison between viral RNA copy number and viral titer showed that the isolate from fetal tissues was produced more efficiently than the fever-associated isolate. Together, these results suggest that different ZIKV isolates differ in their replication capacity, and this might contribute to the fetotropic potential of a particular strain. |
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