Immunomodulatory function of antimicrobial peptide EC-Hepcidin1 modulates the induction of inflammatory gene expression in primary cells of Caspian Trout (Salmo trutta caspius Kessler, 1877).

Autor: Ghodsi Z; Department of Aquaculture, Marine Sciences Faculty, Tarbiat Modares University, Tehran, Iran., Kalbassi MR; Department of Aquaculture, Marine Sciences Faculty, Tarbiat Modares University, Tehran, Iran. Electronic address: kalbassi_m@modares.ac.ir., Farzaneh P; Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran., Mobarez AM; Department of Bacteriology, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran., Beemelmanns C; Leibniz Institute for Natural Product Research and Infection Biology e.V. Hans-Knöll-Institute (HKI), Jena, Germany., Amiri Moghaddam J; Leibniz Institute for Natural Product Research and Infection Biology e.V. Hans-Knöll-Institute (HKI), Jena, Germany. Electronic address: jamshid.amiri-moghaddam@hki-jena.de.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2020 Sep; Vol. 104, pp. 55-61. Date of Electronic Publication: 2020 May 28.
DOI: 10.1016/j.fsi.2020.05.067
Abstrakt: Hepcidins, a group of antimicrobial peptides (AMPs), play a key role in the innate immune system of fishes and act against different pathogens. In this study, antimicrobial and immune-inflammatory activity of a synthetic EC-hepcidin1, previously identified from orange-spotted grouper, were evaluated. EC-hepcidin1 showed weak activity against the zoonotic fish pathogen Streptococcus iniae (MIC 100 μg mL -1 and MBC 150 μg mL -1 ). To study the effect of AMPs in general, and EC-hepcidin1 in particular, a primary cell culture (SC) from the fin tissue of the Caspian Trout (Salmo trutta caspius) was established. The neutral Red method on SC cells revealed that EC-hepcidin1 has no or very low cytotoxic properties. Treatment of cells with either EC-hepcidin1 (150 μg mL -1 ) or fish pathogen Streptococcus iniae (MOI = 10) and a mixture of both resulted in the up-regulation of gene expression of MHC-UBA, IL-6, and TNFα indicating the modulatory function on inflammatory processes. These findings indicate that EC-hepcidin1 might act as a candidate for modulation of the innate immune system in S. iniae-based infection.
(Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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