| Autor: |
Namountougou M; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso.; Institut Supérieur des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso BP 1091, Burkina Faso., Soma DD; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso.; Institut Supérieur des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso BP 1091, Burkina Faso., Balboné M; Département de Biologie et de Physiologie Animales, Université Joseph Ki-Zerbo, Ouagadougou BP 7021, Burkina Faso., Kaboré DA; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Kientega M; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Hien A; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Coulibaly A; Unité de formation et de recherche en Sciences et techniques, Université Norbert Zongo, Koudougou BP 376, Burkina Faso., Ouattara PE; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Meda BG; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Drabo S; Département de Biologie et de Physiologie Animales, Université Joseph Ki-Zerbo, Ouagadougou BP 7021, Burkina Faso., Koala L; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Nignan C; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Kagoné T; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Diabaté A; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso., Fournet F; Maladies Infectieuses et Vecteurs : Écologie et Contrôle (MIVEGEC), Univ Montpellier, CNRS, IRD, 34394 Montpellier, France., Gnankiné O; Département de Biologie et de Physiologie Animales, Université Joseph Ki-Zerbo, Ouagadougou BP 7021, Burkina Faso., Dabiré RK; Institut de Recherche en Sciences de la Santé (IRSS), Bobo-Dioulasso BP 545, Burkina Faso.; Département des Sciences Biomédicales, Centre Muraz, Bobo-Dioulasso BP 390, Burkina Faso. |
| Abstrakt: |
In West Africa, Aedes aegypti remains the major vector of dengue virus. Since 2013, dengue fever has been reemerging in Burkina Faso with annual outbreaks, thus becoming a major public health problem. Its control relies on vector control, which is unfortunately facing the problem of insecticide resistance. At the time of this study, although data on phenotypic resistance were available, information related to the metabolic resistance in Aedes populations from Burkina Faso remained very scarce. Here, we assessed the phenotypic and the metabolic resistance of Ae. aegypti populations sampled from the two main urban areas (Ouagadougou and Bobo-Dioulasso) of Burkina Faso. Insecticide susceptibility bioassays to chlorpyriphos-methyl 0.4%, bendiocarb 0.1% and deltamethrin 0.05% were performed on natural populations of Ae. aegypti using the WHO protocol. The activity of enzymes involved in the rapid detoxification of insecticides, especially non-specific esterases, oxidases (cytochrome P450) and glutathione-S-transferases, was measured on individual mosquitos. The mortality rates for deltamethrin 0.05% were low and ranged from 20.72% to 89.62% in the Bobo-Dioulasso and Ouagadougou sites, respectively. When bendiocarb 0.1% was tested, the mortality rates ranged from 7.73% to 71.23%. Interestingly, in the two urban areas, mosquitoes were found to be fully susceptible to chlorpyriphos-methyl 0.4%. Elevated activity of non-specific esterases and glutathione-S-transferases was reported, suggesting multiple resistance mechanisms involved in Ae. aegypti populations from Bobo-Dioulasso and Ouagadougou (including cytochrome P450). This update to the insecticide resistance status within Ae. aegypti populations in the two biggest cities is important to better plan dengue vectors control in the country and provides valuable information for improving vector control strategies in Burkina Faso, West Africa. |
