Metabolic changes in follicular fluids of patients treated with recombinant versus urinary human chorionic gonadotropin for triggering ovulation in assisted reproductive technologies: a metabolomics pilot study.

Autor: Castiglione Morelli MA; Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100, Potenza, Italy., Iuliano A; Center for Reproductive Medicine of 'San Carlo' Hospital, via Potito Petrone, 85100, Potenza, Italy., Schettini SCA; Center for Reproductive Medicine of 'San Carlo' Hospital, via Potito Petrone, 85100, Potenza, Italy., Petruzzi D; Center for Reproductive Medicine of 'San Carlo' Hospital, via Potito Petrone, 85100, Potenza, Italy., Ferri A; Center for Reproductive Medicine of 'San Carlo' Hospital, via Potito Petrone, 85100, Potenza, Italy., Colucci P; Center for Reproductive Medicine of 'San Carlo' Hospital, via Potito Petrone, 85100, Potenza, Italy., Viggiani L; Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100, Potenza, Italy., Ostuni A; Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100, Potenza, Italy. angela.ostuni@unibas.it.
Jazyk: angličtina
Zdroj: Archives of gynecology and obstetrics [Arch Gynecol Obstet] 2020 Sep; Vol. 302 (3), pp. 741-751. Date of Electronic Publication: 2020 May 27.
DOI: 10.1007/s00404-020-05609-z
Abstrakt: Introduction: The main goal of this retrospective cohort study is the assessment of the effects of administration of recombinant-hCG (r-hCG) versus urinary-hCG (u-hCG) on follicular fluid (FF) composition of women who underwent in vitro fertilization (IVF) treatments.
Materials and Methods: We selected 70 patients with infertility attributable to tubal diseases, unexplained infertility, and male factor. Metabolomics analysis of their FFs was performed by 1 H nuclear magnetic resonance ( 1 H NMR) spectroscopy in combination with multivariate analysis to interpret the spectral data. Univariate statistical analysis was applied to investigate the possible correlations between clinical parameters and between clinical parameters and metabolites identified by NMR.
Results: According to the type of hCG used, significant differences were detected in FFs of women with male factor and unexplained infertility, both in qualitative and quantitative terms, for some metabolites as cholesterol, citrate, creatine, β-hydroxybutyrate, glycerol, lipids, amino acids (Glu, Gln, His, Val, Lys) and glucose. No significant difference was observed in women with tubal diseases. Besides, the number of MII oocytes in the u-hCG-treated groups correlates positively with glutamate in tubal disease and with glycerol in unexplained infertility. In the r-hCG-treated groups, the number of MII oocytes correlates positively with lipid in tubal disease, positively with citrate and negatively with glucose in male infertility.
Conclusions: Metabolite composition of FF changes according to different type of hCG treatment and this can be related to oocyte development and subsequent outcome. According to the data of this study, different types of hCG should be used in relation to the diagnosis of infertility to obtain better results in inducing oocyte maturation in women undergoing IVF.
Databáze: MEDLINE
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