Genotyping on blood and buccal cells using loop-mediated isothermal amplification in healthy humans.

Autor:	Drogou C; Institut de Recherche Biomédicale des Armées (IRBA), Brétigny sur Orge, France.; Université de Paris, Equipe d'Accueil VIgilance FAtigue et SOmmeil (VIFASOM), EA 7330, Paris, France., Sauvet F; Institut de Recherche Biomédicale des Armées (IRBA), Brétigny sur Orge, France.; Université de Paris, Equipe d'Accueil VIgilance FAtigue et SOmmeil (VIFASOM), EA 7330, Paris, France., Erblang M; Institut de Recherche Biomédicale des Armées (IRBA), Brétigny sur Orge, France.; Université de Paris, Equipe d'Accueil VIgilance FAtigue et SOmmeil (VIFASOM), EA 7330, Paris, France., Detemmerman L; LaCAR MDX Technologies, Science Park, Liège, Belgium., Derbois C; Université Paris-Saclay, CEA, Centre National de Recherche en Génomique Humaine (CNRGH), Evry, France., Erkel MC; Institut de Recherche Biomédicale des Armées (IRBA), Brétigny sur Orge, France., Boland A; Université Paris-Saclay, CEA, Centre National de Recherche en Génomique Humaine (CNRGH), Evry, France., Deleuze JF; Université Paris-Saclay, CEA, Centre National de Recherche en Génomique Humaine (CNRGH), Evry, France., Gomez-Merino D; Institut de Recherche Biomédicale des Armées (IRBA), Brétigny sur Orge, France.; Université de Paris, Equipe d'Accueil VIgilance FAtigue et SOmmeil (VIFASOM), EA 7330, Paris, France., Chennaoui M; Institut de Recherche Biomédicale des Armées (IRBA), Brétigny sur Orge, France.; Université de Paris, Equipe d'Accueil VIgilance FAtigue et SOmmeil (VIFASOM), EA 7330, Paris, France.
Jazyk:	angličtina
Zdroj:	Biotechnology reports (Amsterdam, Netherlands) [Biotechnol Rep (Amst)] 2020 May 13; Vol. 26, pp. e00468. Date of Electronic Publication: 2020 May 13 (Print Publication: 2020).
DOI:	10.1016/j.btre.2020.e00468
Abstrakt:	Genetic variations contribute to phenotypic individual vulnerabilities to sleep debt, particularly for five single nucleotide polymorphisms (SNPs). Loop-mediated isothermal amplification and melting curve analysis (LAMP-MC) is a recently developed method to characterize SNPs. The aim of present study was to evaluate the LAMP-MC method on blood and buccal cells for detection of five SNPs of interest in healthy humans. We first analyzed signals obtained from LAMP-MC method on 42 samples. Then we compared the results with those of referent TaqMan method. The LAMP-MC method produced specific melting curves for the five SNPs. A high concordance of genotyping results was observed between the two methods for rs5751876_ADORA2A, rs1800629_TNF-α, rs73598374_ADA and rs228697_PER3 in blood and saliva (Cohen's kappa coefficient >0.80). A good agreement ( = 0.61) was observed for rs4680_COMT in blood only. LAMP-MC is a simple and reliable method to study genetic influences on health, sleep debt-related performance impairments and countermeasures. Competing Interests: L.D. is a full-time employee of LaCAR MDX. Other authors have no competing interests with this study to declare. This study has been funded by the Armaments Procurement Agency (Direction Générale de l'Armement, DGA, Ministry of Armed Forces, contract PDH-1-SMO-2-509). (© 2020 The Authors.)
Databáze:	MEDLINE
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