Unilateral acute anterior uveitis is associated with ipsilateral changes in the tear fluid proteome that involves the LXR/RXR pathway.

Autor: Eidet JR; Center for Eye Research, Department of Ophthalmology, Oslo University Hospital, University of Oslo, Oslo, Norway. j.r.eidet@gmail.com., Jørstad ØK; Center for Eye Research, Department of Ophthalmology, Oslo University Hospital, University of Oslo, Oslo, Norway., Fostad IG; Faculty of Dentistry, Department of Oral Biology, University of Oslo, Oslo, Norway., Olstad OK; Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway., Sørland RØ; Center for Eye Research, Department of Ophthalmology, Oslo University Hospital, University of Oslo, Oslo, Norway., Moe MC; Center for Eye Research, Department of Ophthalmology, Oslo University Hospital, University of Oslo, Oslo, Norway., Petrovski G; Center for Eye Research, Department of Ophthalmology, Oslo University Hospital, University of Oslo, Oslo, Norway., Pepaj M; Hormone Laboratory, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
Jazyk: angličtina
Zdroj: Journal of ophthalmic inflammation and infection [J Ophthalmic Inflamm Infect] 2020 May 27; Vol. 10 (1), pp. 13. Date of Electronic Publication: 2020 May 27.
DOI: 10.1186/s12348-020-00204-4
Abstrakt: Purpose: To determine whether unilateral acute anterior uveitis (AAU) induces ipsilateral changes in the tear fluid proteome.
Methods: Five patients (25-77 years old) with unilateral AAU were included. Tear fluid samples were obtained using Schirmer's test strips. The healthy eye served as control. Proteins were identified by liquid chromatography tandem mass spectrometry.
Results: Two hundred forty-two tear fluid sample proteins were identified, of which 75 were present in at least three patients. Nine proteins were at least 1.5-fold increased, whereas eight were at least 1.5-fold decreased in tears from the diseased eye compared with the healthy eye. APOBEC3A was significantly increased (1.43-fold; P = 0.04), whereas TGM2 was significantly decreased (- 1.21-fold; P = 0.03) in tears from the diseased eye relative to the healthy eye. Ingenuity Pathway Analysis identified LXR/RXR (P < 1.02E-4) as a top canonical pathway.
Conclusion: Unilateral AAU induced detectable changes in the ipsilateral tear fluid proteome and involvement of the inflammation-associated LXR/RXR pathway.
Databáze: MEDLINE
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