Graphene Oxide Nanosheets Tailored With Aromatic Dipeptide Nanoassemblies for a Tuneable Interaction With Cell Membranes.
| Autor: | Trapani G; Scuola Superiore di Catania, University of Catania, Catania, Italy., Caruso VCL; Department of Chemical Sciences, Nano Hybrid BioInterfaces Lab (NHBIL), University of Catania, Catania, Italy., Cucci LM; Department of Chemical Sciences, Nano Hybrid BioInterfaces Lab (NHBIL), University of Catania, Catania, Italy., Attanasio F; Institute of Crystallography - National Council of Research, Catania, Italy., Tabbì G; Institute of Crystallography - National Council of Research, Catania, Italy., Forte G; Department of Pharmaceutical Sciences, University of Catania, Catania, Italy., La Mendola D; Department of Pharmacy, University of Pisa, Pisa, Italy., Satriano C; Department of Chemical Sciences, Nano Hybrid BioInterfaces Lab (NHBIL), University of Catania, Catania, Italy. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2020 May 08; Vol. 8, pp. 427. Date of Electronic Publication: 2020 May 08 (Print Publication: 2020). |
| DOI: | 10.3389/fbioe.2020.00427 |
| Abstrakt: | Engineered graphene-based derivatives are attractive and promising candidates for nanomedicine applications because of their versatility as 2D nanomaterials. However, the safe application of these materials needs to solve the still unanswered issue of graphene nanotoxicity. In this work, we investigated the self-assembly of dityrosine peptides driven by graphene oxide (GO) and/or copper ions in the comparison with the more hydrophobic diphenylalanine dipeptide. To scrutinize the peptide aggregation process, in the absence or presence of GO and/or Cu 2+ , we used atomic force microscopy, circular dichroism, UV-visible, fluorescence and electron paramagnetic resonance spectroscopies. The perturbative effect by the hybrid nanomaterials made of peptide-decorated GO nanosheets on model cell membranes of supported lipid bilayers was investigated. In particular, quartz crystal microbalance with dissipation monitoring and fluorescence recovery after photobleaching techniques were used to track the changes in the viscoelastic properties and fluidity of the cell membrane, respectively. Also, cellular experiments with two model tumour cell lines at a short time of incubation, evidenced the high potential of this approach to set up versatile nanoplatforms for nanomedicine and theranostic applications. (Copyright © 2020 Trapani, Caruso, Cucci, Attanasio, Tabbì, Forte, La Mendola and Satriano.) |
| Databáze: | MEDLINE |
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