| Autor: |
Shamikh YI; Department of Microbiology and Immunology, Nahda University in Beni Suef, Beni-Suef 65211, Egypt.; Consultant, Virology Department, Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo 11517, Egypt., El Shamy AA; Department of Microbiology and Public Health, Faculty of Pharmacy, Heliopolis University for Sustainable Development, Cairo 11785, Egypt., Gaber Y; Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt.; Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Mutah University, Karak 61710, Jordan., Abdelmohsen UR; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, 7 Universities Zone, New Minia 61111, Egypt.; Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha 61441, Saudi Arabia., Madkour HA; Department of Marine and Environmental Geology, National Institute of Oceanography and Fisheries, Red Sea Branch, Hurghada 84511, Egypt., Horn H; Independent Researcher, 69126 Heidelberg, Germany., Hassan HM; Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt., Elmaidomy AH; Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt., Alkhalifah DHM; Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia., Hozzein WN; Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.; Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. |
| Abstrakt: |
The diversity of actinomycetes associated with the marine sponge Coscinoderma mathewsi collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, three isolates were classified as novel species of the genera Micromonospora , Nocardia , and Gordonia . Genome sequencing of actinomycete strains has revealed many silent biosynthetic gene clusters and has shown their exceptional capacity for the production of secondary metabolites, not observed under classical cultivation conditions. Therefore, the effect of mycolic-acid-containing bacteria or mycolic acid on the biosynthesis of cryptic natural products was investigated. Sponge-derived actinomycete Micromonospora sp. UA17 was co-cultured using liquid fermentation with two mycolic acid-containing actinomycetes ( Gordonia sp. UA19 and Nocardia sp. UA 23), or supplemented with pure mycolic acid. LC-HRESIMS data were analyzed to compare natural production across all crude extracts. Micromonospora sp. UA17 was rich with isotetracenone, indolocarbazole, and anthracycline analogs. Some co-culture extracts showed metabolites such as a chlorocardicin, neocopiamycin A, and chicamycin B that were not found in the respective monocultures, suggesting a mycolic acid effect on the induction of cryptic natural product biosynthetic pathways. The antibacterial, antifungal, and antiparasitic activities for the different cultures extracts were also tested. |
