Comparative In Vitro Assessment of the Methanol Extracts of the Leaf, Stem, and Root Barks of Cnidoscolus aconitifolius on Lung and Breast Cancer Cell Lines.

Autor: Oikpefan EO; Delta State University, Faculty of Pharmacy, Department of Pharmacognosy and Traditional Medicine, Abraka, Nigeria., Ayinde BA; University of Benin, Faculty of Pharmacy, Department of Pharmacognosy, Benin City, Nigeria., Mudassir A; University of Karachi, International Centre for Chemical and Biological Sciences, Dr. Panjwani Center for Molecular Medicine and Drug Research, Karachi, Pakistan., Farooq AD; University of Karachi, International Centre for Chemical and Biological Sciences, Dr. Panjwani Center for Molecular Medicine and Drug Research, Karachi, Pakistan.
Jazyk: angličtina
Zdroj: Turkish journal of pharmaceutical sciences [Turk J Pharm Sci] 2019 Dec; Vol. 16 (4), pp. 375-379. Date of Electronic Publication: 2019 Nov 11.
DOI: 10.4274/tjps.galenos.2018.19942
Abstrakt: Objectives: Cnidoscolus aconitifolius Mill. I.M.Johnst. is a medicinal plant widely used in ethnomedicine for the treatment of cancer and other diseases.
Materials and Methods: The effects of methanol extracts of the leaf, stem, and root barks were evaluated on breast (MCF-7) and lung (NCI-H460) cancer cells at 1-250 μg/mL using the SRB assay and the extracts were screened for phytochemicals using the standard method.
Results: The stem and root extracts showed no activity at the maximum concentration, while the leaf extract at 100 μg/mL showed remarkable cell growth inhibition against breast (-14.50±0.58) and lung cancer (+53.29±4.57) in vitro. The extracts showed the presence of saponins, terpenes, cardiac glycosides, and phenolic compounds. Partitioning of the active leaf extract further enhanced its activity as the chloroform fraction exhibited GI 50 , LC 50 , and total growth inhibition (TGI) of 22.5, 68.75, and 43.75 μg/mL against breast cancer, respectively, and GI 50 and TGI of 35.4 and 55.8 μg/ mL against lung cancer cells, respectively. However, the aqueous fraction showed no cytotoxicity against either cell line.
Conclusion: These results justified the ethnomedicinal uses of the plant against tumor-related ailments. Isolation of the constituents responsible for the observed activity needs to be carried out to further support this claim.
Competing Interests: Conflicts of interest: No conflict of interest was declared by the authors.
(©Copyright 2019 Turk J Pharm Sci, Published by Galenos Publishing House.)
Databáze: MEDLINE
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