Fat-1 expression enhance hippocampal memory in scopolamine-induced amnesia.

Autor: Hwang TW; Departments of Medical Science, School of Medicine, Chungnam National University, Daejeon 35015; Departments of Nephrology, School of Medicine, Chungnam National University, Daejeon 35015., Kim EJ; Departments of Medical Science, School of Medicine, Chungnam National University, Daejeon 35015; Departments of Nephrology, School of Medicine, Chungnam National University, Daejeon 35015., Kim D; Departments of Medical Science, School of Medicine, Chungnam National University, Daejeon 35015; Departments of Nephrology, School of Medicine, Chungnam National University, Daejeon 35015., Jeong JY; Departments of Medical Science, School of Medicine, Chungnam National University, Daejeon 35015; Departments of Nephrology, School of Medicine, Chungnam National University, Daejeon 35015., Kim GH; Drug & Disease Target Team, Division of Bioconvergence Analysis, Republic of Korea, Basic Science Institute, Cheongju 28119., Lim K; Biochemistry, School of Medicine, Chungnam National University, Daejeon 35015., Moon M; Department of Biochemistry, College of Medicine, Konyang University, Daejeon, 35365., Yoon KA; Department of Clinical Laboratory Science, Daejeon Health Sciences College, Daejeon, 34504., Choi DE; Departments of Nephrology, School of Medicine, Chungnam National University, Daejeon 35015. Electronic address: daenii123@gmail.com., Kim JJ; Departments of Medical Science, School of Medicine, Chungnam National University, Daejeon 35015; Departments of Nephrology, School of Medicine, Chungnam National University, Daejeon 35015. Electronic address: kjj1021@naver.com.
Jazyk: angličtina
Zdroj: The Journal of nutritional biochemistry [J Nutr Biochem] 2020 Aug; Vol. 82, pp. 108394. Date of Electronic Publication: 2020 Apr 08.
DOI: 10.1016/j.jnutbio.2020.108394
Abstrakt: Omega-3 polyunsaturated fatty acids (PUFA) are critical for optimal brain health and are involved in psychiatric and neurological ailments. Here, we report the effects of higher endogenous omega-3 PUFA on memory impairment in the hippocampus by studying mice with transgenic expression of the fat-1 gene that converts omega-6 to omega-3 PUFA. We performed Y-maze and passive avoidance tests to evaluate the memory function of fat-1 mice treated with scopolamine. Fat-1 mice showed induced alternation in the Y-maze test and increased latency in the passive avoidance test. The effects of scopolamine on hippocampal neurogenesis were confirmed by increases in the number of Ki-67- and DCX-positive cells in the fat-1 mice. Western blotting revealed increased brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element-binding protein levels, and lower scopolamine-induced apoptosis based on the cleaved-caspase 3 protein level in fat-1 mice. These findings suggest that higher endogenous omega-3 PUFA prevented granular cell loss, increased BDNF signaling, and decreased apoptosis signaling in scopolamine-treated fat-1 mice. These processes may underlie granular cell survival and suggest potential therapeutic targets for memory impairment.
Competing Interests: Declaration of competing interest The authors declare no competing financial interests.
(Copyright © 2020. Published by Elsevier Inc.)
Databáze: MEDLINE
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