Fast and reversible neural inactivation in macaque cortex by optogenetic stimulation of GABAergic neurons.
| Autor: | De A; Graduate Program in Neuroscience, University of Washington, Seattle, United States.; Department of Physiology and Biophysics, Washington National Primate Research Center, University of Washington, Seattle, United States., El-Shamayleh Y; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, United States., Horwitz GD; Department of Physiology and Biophysics, Washington National Primate Research Center, University of Washington, Seattle, United States. |
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| Jazyk: | angličtina |
| Zdroj: | ELife [Elife] 2020 May 26; Vol. 9. Date of Electronic Publication: 2020 May 26. |
| DOI: | 10.7554/eLife.52658 |
| Abstrakt: | Optogenetic techniques for neural inactivation are valuable for linking neural activity to behavior but they have serious limitations in macaques. To achieve powerful and temporally precise neural inactivation, we used an adeno-associated viral (AAV) vector carrying the channelrhodopsin-2 gene under the control of a Dlx5/6 enhancer, which restricts expression to GABAergic neurons. We tested this approach in the primary visual cortex, an area where neural inactivation leads to interpretable behavioral deficits. Optical stimulation modulated spiking activity and reduced visual sensitivity profoundly in the region of space represented by the stimulated neurons. Rebound firing, which can have unwanted effects on neural circuits following inactivation, was not observed, and the efficacy of the optogenetic manipulation on behavior was maintained across >1000 trials. We conclude that this inhibitory cell-type-specific optogenetic approach is a powerful and spatiotemporally precise neural inactivation tool with broad utility for probing the functional contributions of cortical activity in macaques. Competing Interests: AD, YE, GH No competing interests declared (© 2020, De et al.) |
| Databáze: | MEDLINE |
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