Molecular Detection of SARS-CoV-2 Infection in FFPE Samples and Histopathologic Findings in Fatal SARS-CoV-2 Cases.

Autor:	Sekulic M; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.; Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH., Harper H; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.; Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH., Nezami BG; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.; Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH., Shen DL; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.; Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH., Sekulic SP; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.; Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH., Koeth AT; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH., Harding CV; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.; Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH., Gilmore H; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.; Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH., Sadri N; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH.; Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH.
Jazyk:	angličtina
Zdroj:	American journal of clinical pathology [Am J Clin Pathol] 2020 Jul 07; Vol. 154 (2), pp. 190-200.
DOI:	10.1093/ajcp/aqaa091
Abstrakt:	Objectives: To report methods and findings of 2 autopsies with molecular evaluation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive individuals. Methods: Postmortem examination was completed following Centers for Disease Control and Prevention public guidelines. Numerous formalin-fixed paraffin-embedded (FFPE) tissue types from each case were surveyed for SARS-CoV-2 RNA by quantitative reverse transcription polymerase chain reaction (qRT-PCR). SARS-CoV-2 viral genome was sequenced by next-generation sequencing (NGS) from FFPE lung tissue blocks. Results: Postmortem examinations revealed diffuse alveolar damage, while no viral-associated hepatic, cardiac, or renal damage was observed. Viral RNA was detected in lungs, bronchi, lymph nodes, and spleen in both cases using qRT-PCR method. RNA sequencing using NGS in case 1 revealed mutations most consistent with Western European Clade A2a with ORF1a L3606F mutation. Conclusions: SARS-CoV-2 testing and viral sequencing can be performed from FFPE tissue. Detection and sequencing of SARS-CoV-2 in combination with morphological findings from postmortem tissue examination can aid in gaining a better understanding of the virus's pathophysiologic effects on human health. (© American Society for Clinical Pathology, 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu