Genome to phenome tools: In vivo and in vitro transfection of Crassostrea virginica hemocytes.

Autor: Tracy AN; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, 04544, USA; Colby College, Waterville, 4,000 Mayflower Hill Dr, ME, 04901, USA., Yadavalli R; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, 04544, USA., Reed KS; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, 04544, USA; Colby College, Waterville, 4,000 Mayflower Hill Dr, ME, 04901, USA., Parnaik R; North Cornwall Research Institute, Bude, Cornwall, EX23 9EE, UK., Poulton NJ; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, 04544, USA., Bishop-Bailey D; North Cornwall Research Institute, Bude, Cornwall, EX23 9EE, UK., Fernández Robledo JA; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, 04544, USA. Electronic address: jfernandez-robledo@bigelow.org.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2020 Aug; Vol. 103, pp. 438-441. Date of Electronic Publication: 2020 May 23.
DOI: 10.1016/j.fsi.2020.05.022
Abstrakt: The sequencing of the Crassostrea virginica genome has brought back the interest for gene delivery and editing methodologies. Here, we report the expression in oyster hemocytes of two heterologous expression vectors under the CMV promoter delivered with dendrimers. Expression was monitored using confocal microscopy, flow cytometry, and immunofluorescence assay. C. virginica hemocytes were able to express the green fluorescence protein and Crassostrea gigas vascular endothelial growth factor under CMV viral promoter both in vivo and in vitro. These results provide the bases for interrogating the genome and adapting genome editing methodologies.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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