High Molecular Weight Poly(ethylenimine)-Based Water-Soluble Lipopolymer for Transfection of Cancer Cells.

Autor: Abd Elhameed HAH; Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, Szeged, H-6720, Hungary., Ungor D; Interdisciplinary Excellence Centre, Department of Physical Chemistry and Materials Science, University of Szeged, Rerrich Béla tér 1, Szeged, H-6720, Hungary., Igaz N; Department of Biochemistry and Molecular Biology Doctoral School of Biology, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary., Gopisetty MK; Department of Biochemistry and Molecular Biology Doctoral School of Biology, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary., Kiricsi M; Department of Biochemistry and Molecular Biology Doctoral School of Biology, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary., Csapó E; Interdisciplinary Excellence Centre, Department of Physical Chemistry and Materials Science, University of Szeged, Rerrich Béla tér 1, Szeged, H-6720, Hungary.; Faculty of Medicine, MTA-SZTE Biomimetic Systems Research Group, Department of Medical Chemistry, University of Szeged, Dóm tér 8, Szeged, H-6720, Hungary., Gyurcsik B; Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, Szeged, H-6720, Hungary.
Jazyk: angličtina
Zdroj: Macromolecular bioscience [Macromol Biosci] 2020 Jul; Vol. 20 (7), pp. e2000040. Date of Electronic Publication: 2020 May 25.
DOI: 10.1002/mabi.202000040
Abstrakt: Over the past decade, search for novel materials for nucleic acid delivery has prompted a special interest in polymeric nanoparticles (NPs). In this study, the biological applicability of a water-soluble cationic lipopolymer (WSLP) obtained by the modification of high molecular weight branched poly(ethylenimine) (PEI) with cholesteryl chloroformate is characterized and assessed for better cellular membrane permeability. To test the delivery efficiency of the produced lipopolymer, plasmid DNA (pDNA) encoding the enhanced green fluorescent protein and WSLP are mixed at different charge ratios. WSLP and WSLP/pDNA complexes are characterized by dynamic and static light scattering, particle charge detection, scanning electron microscopy, and transmission electron microscopy. The pDNA loading of WSLP is also verified by agarose gel electrophoresis. Cytotoxicity of PEI, WSLP, and of WSLP/pDNA is evaluated on human A549 and HeLa cells. A remarkable dependence of the toxicity on the dose, cholesterylation, and charge ratio is detected. Transfection is monitored by flow cytometry and by fluorescence microscopy. Importantly, cholesterylation decreases the toxicity of the polymer, while promoting high transfection efficiency in both cell lines. This work indicates a possible optimization mode of the high molecular weight PEI-based WSLP rendering it a promising candidate for gene delivery.
(© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
Externí odkaz: