| Autor: |
de Oliveira Filho JWG; Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí-IFPI, Teresina, Brazil., Andrade TJADS; Nucleus of Applied Research to Sciences (NIAC), Federal Institute of Education, Science and Technology of Maranhão-IFMA, São Luís, Brazil., de Lima RMT; Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí-IFPI, Teresina, Brazil., Silva DHS; Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Products (NuBBE), Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, Brazil., Dos Reis AC; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil., Santos JVO; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil., de Meneses APM; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil., de Carvalho RM; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil., da Mata AMO; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil., de Alencar MVOB; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil., Dias ACS; Laboratory of Genetics and Molecular Biology, Federal University of Maranhão, São Luís, Brazil., da Silva FCC; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil., Islam MT; Laboratory of Theoretical and Computational Biophysics, Ton Duc Thang University, Ho Chi Minh City, Vietnam.; Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam., Clark CCT; Centre for Sport, Exercise, and Life Sciences, Coventry University, Coventry, UK., Sousa JMCE; Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí-IFPI, Teresina, Brazil.; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil., Melo-Cavalcante AAC; Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí-IFPI, Teresina, Brazil.; Laboratory of Research in Toxicological Genetics-LAPGENIC, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil. |
| Abstrakt: |
Endophytic fungi are promising sources of bioactive substances; however, their secondary metabolites are toxic to plants, animals, and humans. This study aimed toevaluate the toxic, cytotoxic, mutagenic and oxidant/antioxidant activities of acetonitrile extract (AEPc), citrinin (CIT) and dicitrinin-A (DIC-A) of Penicillium citrinum . For this, the test substances at 0.5; 1.0; 1.5 and 2 μg/mLwere exposed for 24 and 48 h in Artemia salina , and 48 h in Allium cepa test systems. The oxidant/antioxidant test was evaluated in pre-, co- and post-treatment with the stressor hydrogen peroxide (H 2 O 2 ) in Saccharomyces cerevisiae . The results suggest that the AEPc, CIT and DIC-A at 0.5; 1.0; 1.5 and 2 μg/mL showed toxicity in A. saline , with LC 50 (24 h) of 2.03 μg/mL, 1.71 μg/mL and 2.29 μg/mL, and LC 50 (48 h) of 0.51 μg/mL, 0.54 μg/mL and 0.54 μg/mL, respectively.In A. cepa , the test substances also exerted cytotoxic and mutagenic effects. The AEPc, CIT and DIC-A at lower concentrations modulated the damage induced by H 2 O 2 in the proficient and mutant strains of S. cerevisiae for cytoplasmic and mitochondrial superoxide dismutase. Moreover, the AEPc at 2 μg/mL and CIT at the two highest concentrations did not affect the H 2 O 2 -induced DNA damage in the test strains. In conclusion, AEPc, CIT and DIC-A of P. citrinum may exert their toxic, cytotoxic and mutagenic effects in the test systems possibly through oxidative stress induction pathway. |
