Quantification of homocysteine thiolactone in human saliva and urine by gas chromatography-mass spectrometry.

Autor: Piechocka J; Department of Environmental Chemistry, University of Lodz, Faculty of Chemistry, Łódź, Poland. Electronic address: justyna.piechocka@chemia.uni.lodz.pl., Wrońska M; Department of Environmental Chemistry, University of Lodz, Faculty of Chemistry, Łódź, Poland., Chwatko G; Department of Environmental Chemistry, University of Lodz, Faculty of Chemistry, Łódź, Poland., Jakubowski H; Department of Biochemistry and Biotechnology, University of Life Sciences, Poznań, Poland; Department of Microbiology, Biochemistry and Molecular Genetics,Rutgers-New Jersey Medical School, International Center from Public Health, Newark, NJ, USA., Głowacki R; Department of Environmental Chemistry, University of Lodz, Faculty of Chemistry, Łódź, Poland. Electronic address: rafal.glowacki@chemia.uni.lodz.pl.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2020 Jul 15; Vol. 1149, pp. 122155. Date of Electronic Publication: 2020 May 15.
DOI: 10.1016/j.jchromb.2020.122155
Abstrakt: Homocysteine thiolactone (HTL) is a chemically reactive thioester that has been implicated in cardiovascular disease. So far, its presence has been documented in human and mouse plasma and urine. Here, using a new method, we show that HTL is present in human saliva. The assay involves chloroform-methanol extraction of HTL, lyophilization, and derivatization with N-trimethylsilyl-N-methyl trifluoroacetamide (MSTFA) and trimethylchlorosilane (TMCS). The method is based on a gas chromatography coupled with mass spectrometry (GC-MS) and quantifies HTL in a linear range from 0.05 to 1 µmol L -1 saliva and urine. The limit of quantification (LOQ) was 0.05 µmol L -1 . With respect to saliva specimen, the accuracy was 98.7-112.6%, and 90.2-100.5%, while the precision was 7.1-13.5% and 12.5-15.0% for the intra- and inter-day variation, respectively. In relation to urine samples, the accuracy was 91.9-110.9% and 91.2-103.3%, while the precision varied from 2.2% to 14.5% and 7.4% to 14.3% for intra- and inter-day measurements, respectively. Using this method, we show that in apparently healthy individuals (n = 18), HTL levels in saliva are not positively correlated with urinary HTL levels. Undoubtedly, larger population should be investigated to get more meaningful results.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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