In vitro and in vivo impairment of embryo implantation by commonly used fungicide Mancozeb.

Autor: Akthar I; Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka; Postgraduate Institute of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka., Wang Z; Department of Obstetrics and Gynaecology LKS Faculty of Medicine, The University of Hong Kong, HKSAR, China., Wijayagunawardane MPB; Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka; Postgraduate Institute of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka., Ratnayake CJ; Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Peradeniya, Peradeniya, 20400, Sri Lanka., Siriweera EH; Department of Pathology, Faculty of Medicine, University of Peradeniya, Peradeniya, 20400, Sri Lanka., Lee KF; Department of Obstetrics and Gynaecology LKS Faculty of Medicine, The University of Hong Kong, HKSAR, China; Shenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong Shenzhen Hospital, The University of Hong Kong, Shenzhen, China. Electronic address: ckflee@hku.hk., Kodithuwakku SP; Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka; Postgraduate Institute of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka. Electronic address: surangap@agri.pdn.ac.lk.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Jun 18; Vol. 527 (1), pp. 42-48. Date of Electronic Publication: 2020 Apr 24.
DOI: 10.1016/j.bbrc.2020.04.051
Abstrakt: The fungicide Mancozeb is an endocrine-disrupting chemical and the mode of action of Mancozeb on embryo implantation is largely unknown. Mancozeb (1 and 3 μg/ml) significantly reduced Jeg-3 trophoblastic spheroids attachment to endometrial epithelial Ishikawa cells. Mancozeb treatment from gestation day (GD) 1 to GD8 or from GD4 to GD8 significantly lowered the number of implantation sites with higher incidence of morphological abnormalities in the reproductive tissues. However, these were not seen in the treatment from GD1 to GD4. Mancozeb at 30 mg/kg BW/d did not alter the expression of p53, COX-2, or PGFS transcripts in the uterus, but down-regulated the PGES transcript and protein. Mancozeb treatment in human endometrial stromal cells did not alter the decidualization response, but the morphological transformation was impaired. Taken together, exposure to Mancozeb affected embryo implantation probably through the modulation of decidualization and to delineate the exact mode of action needs further investigations.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE