Paired wavelength relevance as spectrophotometric strategy for evaluation the potency of medicine affecting human health.

Autor: Lotfy HM; Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, 11562 Cairo, Egypt; Pharmaceutical Chemistry Department, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University, 12311 Cairo, Egypt., Fayez YM; Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, 11562 Cairo, Egypt., Michael AM; Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 12566 6th of October City, Egypt., Monir HH; Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, 11562 Cairo, Egypt., Nessim CK; Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 12566 6th of October City, Egypt. Electronic address: kokykemo49@yahoo.com.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2020 Oct 05; Vol. 239, pp. 118461. Date of Electronic Publication: 2020 May 11.
DOI: 10.1016/j.saa.2020.118461
Abstrakt: Paired wavelength relevance as spectrophotometric strategy was carried for simultaneous analysis of unresolved bands of doxycycline hydrochloride (DOX) and tylosin tartrate (TYT) as an example of veterinary binary mixture. These methods based on the relation between two points on the scanned or manipulated spectra (derivative, ratio). The methods using the absorbance difference between two wavelengths namely, dual wavelength (DW), induced dual wavelength (IDW), absorption correction (AC) and advanced absorbance subtraction (AAS) or amplitude difference namely, ratio difference (RD), amplitude subtraction namely, amplitude correction (PC) and advanced amplitude modulation (AAM) as well as amplitude addition namely, derivative ratio (DD 1 ). Calibration graphs are direct relation in the range of (5-40) μg/mL for doxycycline hydrochloride and (5-45) μg/mL for tylosin tartrate. The suggested methods was successfully applied for evaluate the potency of their veterinary medicine that has direct affecting human health. The method has been validated according to the guideline of international conference on Harmonization and parameters like linearity, range, accuracy and precision have been studied. The outcomes were matched statistically with those of the authorized systems; Student's t-test, F-test, and One-way ANOVA, presenting acceptable values and no momentous change with accuracy. The procedures can be considered useful for the quantitative analysis.
Competing Interests: Declaration of competing interest The all authors have no conflict of interest, no significant competing financial, professional, or personal interests that might have influenced the performance or presentation of the work described in this manuscript. All ethical guidelines have been adhered and followed.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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