Inactivation of specific spoilage organism (Pseudomonas) of sturgeon by curcumin-mediated photodynamic inactivation.
Autor: | Gong C; College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Shinan District, 266003 Qingdao, China., Li Y; College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Shinan District, 266003 Qingdao, China., Gao R; School of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Xiao F; College of Food and Bioengineering, Henan University of Science and Technology, 471023 Luoyang, China., Zhou X; Hisense (Shandong) Refrigerator Co., Ltd, 266100 Qingdao, China., Wang H; Hisense (Shandong) Refrigerator Co., Ltd, 266100 Qingdao, China., Xu H; Jiangsu Baoyuan Biotechnology Co., Ltd., 222100 Lianyungang, China., Wang R; College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Shinan District, 266003 Qingdao, China., Huang P; College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Shinan District, 266003 Qingdao, China., Zhao Y; College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Shinan District, 266003 Qingdao, China. Electronic address: zhaoyuanhui@ouc.edu.cn. |
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Jazyk: | angličtina |
Zdroj: | Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2020 Sep; Vol. 31, pp. 101827. Date of Electronic Publication: 2020 May 21. |
DOI: | 10.1016/j.pdpdt.2020.101827 |
Abstrakt: | The present study aimed to measure the inactivation effect and mechanism of curcumin-mediated photodynamic inactivation (PDI) on the specific spoilage organism (Pseudomonas) of the sturgeon. The conditions of PDI used were as follows: 30 μM curcumin, 15 W LED light (470 nm) power and 90 s irradiation time. Under these conditions, the high-throughput sequencing was used to study the microbiota of sturgeon. The method of aerobic plate colony count (APC) was used to determine the viability of Pseudomonas after PDI. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the propidium iodide (PI) single staining method, and agarose gel electrophoresis were used to study the inactivation mechanism of PDI on Pseudomonas. The results showed that Pseudomonas was the specific spoilage organism of sturgeon, and PDI significantly inhibited the growth of Pseudomonas. The in-vitro inactivation rate of Pseudomonas was 99.9% with counts decreased by 3.19 ± 0.15 log (Copyright © 2020 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
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