Infection and microbial molecular motifs modulate transcription of the interferon-inducible gene ifit5 in a teleost fish.

Autor: Bela-Ong DB; The Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries, and Economics, University of Tromsø, The Arctic University of Norway, N-9037, Tromsø, Norway., Greiner-Tollersrud L; The Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries, and Economics, University of Tromsø, The Arctic University of Norway, N-9037, Tromsø, Norway., Andreas van der Wal Y; The Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries, and Economics, University of Tromsø, The Arctic University of Norway, N-9037, Tromsø, Norway; Vaxxinova Research &Development GmBH, Münster, Germany., Jensen I; The Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries, and Economics, University of Tromsø, The Arctic University of Norway, N-9037, Tromsø, Norway., Seternes OM; Department of Pharmacy, University of Tromsø, The Arctic University of Norway, N-9037, Tromsø, Norway., Jørgensen JB; The Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries, and Economics, University of Tromsø, The Arctic University of Norway, N-9037, Tromsø, Norway. Electronic address: jorunn.jorgensen@uit.no.
Jazyk: angličtina
Zdroj: Developmental and comparative immunology [Dev Comp Immunol] 2020 Oct; Vol. 111, pp. 103746. Date of Electronic Publication: 2020 May 21.
DOI: 10.1016/j.dci.2020.103746
Abstrakt: Interferon-induced proteins with tetratricopeptide repeats (IFITs) are involved in antiviral defense. Members of this protein family contain distinctive multiple structural motifs comprising tetratricopeptides that are tandemly arrayed or dispersed along the polypeptide. IFIT-encoding genes are upregulated by type I interferons (IFNs) and other stimuli. IFIT proteins inhibit virus replication by binding to and regulating the functions of cellular and viral RNA and proteins. In teleost fish, knowledge about genes and functions of IFITs is currently limited. In the present work, we describe an IFIT5 orthologue in Atlantic salmon (SsaIFIT5) with characteristic tetratricopeptide repeat motifs. We show here that the gene encoding SsaIFIT5 (SsaIfit5) was ubiquitously expressed in various salmon tissues, while bacterial and viral challenge of live fish and in vitro stimulation of cells with recombinant IFNs and pathogen mimics triggered its transcription. The profound expression in response to various immune stimulation could be ascribed to the identified IFN response elements and binding sites for various immune-relevant transcription factors in the putative promoter of the SsaIfit5 gene. Our results establish SsaIfit5 as an IFN-stimulated gene in A. salmon and strongly suggest a phylogenetically conserved role of the IFIT5 protein in antimicrobial responses in vertebrates.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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