Modulation of the Drug Resistance by Platonia insignis Mart. Extract, Ethyl Acetate Fraction and Morelloflavone/Volkensiflavone (Biflavonoids) in Staphylococcus aureus Strains Overexpressing Efflux Pump Genes.

Autor:	E Silva AKF; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina PI, Brazil., Dos Reis AC; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina PI, Brazil., Pinheiro EEA; Laboratory of Organic Geochemistry, Federal University of Piaui, Teresina, PI, Brazil., de Sousa JN; Laboratory of Research in Microbiology, Federal University of Piaui, Teresina PI, Brazil., de Alcântara Oliveira FA; Laboratory of Research in Microbiology, Federal University of Piaui, Teresina PI, Brazil., Moura AKS; Laboratory of Organic Geochemistry, Federal University of Piaui, Teresina, PI, Brazil., de Sousa L Neto J; Laboratory of Organic Geochemistry, Federal University of Piaui, Teresina, PI, Brazil., das Graças L Citó AM; Laboratory of Organic Geochemistry, Federal University of Piaui, Teresina, PI, Brazil., Siqueira-Júnior JP; Laboratory of Genetic of Microorganisms, Federal University of Paraiba, Joao Pessoa PB, Brazil., Kaatz GW; Department of Medicine, Division of Infectious Diseases, Wayne State University, School of Medicine, Detroit, Michigan, SC, United States., Barreto HM; Laboratory of Research in Microbiology, Federal University of Piaui, Teresina PI, Brazil.
Jazyk:	angličtina
Zdroj:	Current drug metabolism [Curr Drug Metab] 2021; Vol. 22 (2), pp. 114-122.
DOI:	10.2174/1389200221666200523155617
Abstrakt:	Background: Microbial resistance to antibiotics is a global public health problem, which requires urgent attention. Platonia insignis is a native species from the eastern Brazilian Amazon, used in the treatment of burns and wounds. Objectives: To evaluate the antimicrobial activity of the hydroalcoholic extract of P. insignis (PIHA), the ethyl acetate fraction (PIAE), and its subfraction containing a mixture of biflavonoids (BF). Moreover, the effect of these natural products on the antibiotic activity against S. aureus strains overexpressing efflux pump genes was also evaluated. Methods: Minimal inhibitory concentrations were determined against different species of microorganisms. To evaluate the modulatory effect on the Norfloxacin-resistance, the MIC of this antibiotic was determined in the absence and presence of the natural products at subinhibitory concentrations. Inhibition of the EtBr efflux assays were conducted in the absence or presence of natural products. Results: PIHA showed a microbicidal effect against S. aureus and C. albicans, while PIAE was bacteriostatic for S. aureus. PIAE and BF at subinhibitory concentrations were able to reduce the MIC of Norfloxacin acting as modulating agents. BF was able to inhibit the efflux of EtBr efflux in S. aureus strains overexpressing specific efflux pump genes. Conclusion: P. inignisis, a source of efflux pump inhibitors, including volkensiflavone and morelloflavone, which were able to potentiate the Norfloxacin activity by NorA inhibition, being also able to inhibit QacA/B, TetK and MsrA. Volkensiflavone and morelloflavone could be used as an adjuvant in the antibiotic therapy of multidrug resistant S. aureus strains overexpressing efflux pumps. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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