Cell fusing agent virus (Flavivirus) infection in Aedes aegypti in Texas: seasonality, comparison by trap type, and individual viral loads.

Autor: Martin E; Department of Entomology, Texas A&M University, College Station, TX, USA. estellemartin@ufl.edu.; Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA. estellemartin@ufl.edu., Tang W; Department of Entomology, Texas A&M University, College Station, TX, USA., Briggs C; Department of Entomology, Texas A&M University, College Station, TX, USA., Hopson H; Department of Entomology, Texas A&M University, College Station, TX, USA., Juarez JG; Department of Entomology, Texas A&M University, College Station, TX, USA., Garcia-Luna SM; Department of Entomology, Texas A&M University, College Station, TX, USA., de Valdez MW; Department of Science and Mathematics, Texas A&M University-San Antonio, San Antonio, TX, USA., Badillo-Vargas IE; Department of Entomology, Texas A&M AgriLife Research, Weslaco, TX, USA., Borucki MK; Lawrence Livermore National Laboratory, Livermore, CA, USA., Frank M; Lawrence Livermore National Laboratory, Livermore, CA, USA., Hamer GL; Department of Entomology, Texas A&M University, College Station, TX, USA. ghamer@tamu.edu.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2020 Aug; Vol. 165 (8), pp. 1769-1776. Date of Electronic Publication: 2020 May 21.
DOI: 10.1007/s00705-020-04652-0
Abstrakt: South Texas has experienced local transmission of Zika virus and of other mosquito-borne viruses such as chikungunya virus and dengue virus in the last decades. Using a mosquito surveillance program in the Lower Rio Grande Valley (LRGV) and San Antonio, TX, from 2016 to 2018, we detected the presence of an insect-specific virus, cell fusing agent virus (CFAV), in the Aedes aegypti mosquito population. We tested 6,326 females and 1,249 males from the LRGV and 659 females from San Antonio for CFAV by RT-PCR using specific primers. Infection rates varied from 0 to 261 per 1,000 mosquitoes in the LRGV and 115 to 208 per 1,000 in San Antonio depending on the month of collection. Infection rates per 1,000 individuals appeared higher in females collected from BG Sentinel 2 traps compared to Autocidal Gravid Ovitraps, but the ratio of the percentage of infected pools did not differ by trap type. The natural viral load in individual males ranged from 1.25 x 10 2 to 5.50 x 10 6 RNA copies and in unfed females from 5.42 x 10 3 to 8.70 x 10 6 RNA copies. Gravid females were found to harbor fewer viral particles than males and unfed females.
Databáze: MEDLINE