Application of lipase immobilized on a hydrophobic support for the synthesis of aromatic esters.

Autor: Dos Santos MMO; Department of Exact Sciences and Natural, State University of Southwest Bahia, Itapetinga, Brazil., Gama RS; Institute of Chemistry, Federal University of Alfenas, Alfenas, Brazil., de Carvalho Tavares IM; Department of Exact Sciences and Natural, State University of Southwest Bahia, Itapetinga, Brazil., Santos PH; Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianópolis, Brazil., Gonçalves MS; Department of Exact Sciences and Natural, State University of Southwest Bahia, Itapetinga, Brazil., de Carvalho MS; Department of Exact Sciences and Technology, State University of Santa Cruz, Ilhéus, Brazil., de Barros Vilas Boas EV; Department of Food Science, Federal University of Lavras, Lavras, Brazil., de Oliveira JR; Department of Exact Sciences and Technology, State University of Santa Cruz, Ilhéus, Brazil., Mendes AA; Institute of Chemistry, Federal University of Alfenas, Alfenas, Brazil., Franco M; Department of Exact Sciences and Technology, State University of Santa Cruz, Ilhéus, Brazil.
Jazyk: angličtina
Zdroj: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2021 Jun; Vol. 68 (3), pp. 538-546. Date of Electronic Publication: 2020 Jun 15.
DOI: 10.1002/bab.1959
Abstrakt: The present study aimed at preparing three biocatalysts via physical adsorption of lipases from Candida rugosa (CRL), Mucor javanicus, and Candida sp. on a hydrophobic and mesoporous support (Diaion HP-20). These biocatalysts were later applied to the synthesis of aromatic esters of apple peel and citrus (hexyl butyrate), apple and rose (geranyl butyrate), and apricot and pineapple (propyl butyrate). Scanning electron microscopy and gel electrophoresis confirmed a selective adsorption of lipases on Diaion, thus endorsing simultaneous immobilization and purification. Gibbs free energy (∆G) evinced the spontaneity of the process (-17.9 kJ/mol ≤ ∆G ≤ -5.1 kJ/mol). Maximum immobilized protein concentration of 30 mg/g support by CRL. This biocatalyst was the most active in olive oil hydrolysis (hydrolytic activity of 126.0 ± 2.0 U/g) and in the synthesis of aromatic esters. Maximum conversion yield of 89.1% was attained after 150 Min for the synthesis of hexyl butyrate, followed by the synthesis of geranyl butyrate (87.3% after 240 Min) and propyl butyrate (80.0% after 150 Min). CRL immobilized on Diaion retained around 93% of its original activity after six consecutive cycles of 150 Min for the synthesis of hexyl butyrate.
(© 2020 International Union of Biochemistry and Molecular Biology, Inc.)
Databáze: MEDLINE
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