Anethole Supplementation During Oocyte Maturation Improves In Vitro Production of Bovine Embryos.

Autor: Sá NAR; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceara, Av Dr Silas Munguba, 1700, Campus do Itaperi, Fortaleza, Ceara, Brazil., Vieira LA; Department of Physiology, Veterinary Faculty, University of Murcia, Murcia, Spain., Ferreira ACA; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceara, Av Dr Silas Munguba, 1700, Campus do Itaperi, Fortaleza, Ceara, Brazil., Cadenas J; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceara, Av Dr Silas Munguba, 1700, Campus do Itaperi, Fortaleza, Ceara, Brazil., Bruno JB; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceara, Av Dr Silas Munguba, 1700, Campus do Itaperi, Fortaleza, Ceara, Brazil., Maside C; Department of Physiology, Veterinary Faculty, University of Murcia, Murcia, Spain., Sousa FGC; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceara, Av Dr Silas Munguba, 1700, Campus do Itaperi, Fortaleza, Ceara, Brazil., Cibin FWS; Laboratory of Reproduction Biotechnology (Biotech), Campus Uruguaiana, Federal University of Pampa, Uruguaiana, Rio Grande do Sul, Brazil., Alves BG; Laboratory of Reproductive Biology, Institute of Biomedical Sciences, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil., Rodrigues APR; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceara, Av Dr Silas Munguba, 1700, Campus do Itaperi, Fortaleza, Ceara, Brazil., Leal-Cardoso JH; Laboratory of Electrophysiology (LEF), Superior Institute of Biomedical Sciences, State University of Ceara, Fortaleza, Ceara, Brazil., Gastal EL; Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA., Figueiredo JR; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceara, Av Dr Silas Munguba, 1700, Campus do Itaperi, Fortaleza, Ceara, Brazil. jrf.lamofopapapers@gmail.com.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2020 Aug; Vol. 27 (8), pp. 1602-1608.
DOI: 10.1007/s43032-020-00190-x
Abstrakt: Oxidative stress is one of the most detrimental factors that affect oocyte developmental competence and embryo development in vitro. The impact of anethole supplementation to in vitro maturation (IVM) media on oocyte maturation and further bovine in vitro embryo production was investigated. Oocytes of slaughterhouse-derived bovine ovaries were placed in IVM with anethole at different concentrations of 30 (AN30), 300 (AN300), and 2000 μg/mL (AN2000), or without (control treatment). The oocytes were assessed for maturation rates, and for reactive oxygen species (ROS) and ferric reducing antioxidant power (FRAP) levels, and mitochondrial membrane potential. Embryo development was assessed by cleavage and blastocyst rates, and embryo cell number. The percentage of metaphase II oocytes were similar among the treatments (range, 77%-96%). Anethole at 300 μg/mL was the only treatment that yielded higher cleavage and embryo development (morula and blastocyst) rates compared to the control treatment. The ROS production in the oocytes after maturation did not differ among treatments. However, oocytes treated with anethole at 300 μg/mL had higher (P < .05) FRAP and mitochondrial membrane potential compared to the control treatment. Furthermore, AN300 treatment increased (P < .05) the average number of total cells in blastocysts compared to the control and AN30 treatments. The use of anethole at 300 μg/mL during IVM is suggested to improve the quantity and quality of bovine embryos produced in vitro. The beneficial effects of anethole on embryonic developmental competence in vitro seems to be related to its capacity to regulate the redox balance and improve mitochondrial function in oocytes and embryos.
Databáze: MEDLINE
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