Nociceptive responses in melatonin MT 2 receptor knockout mice compared to MT 1 and double MT 1 /MT 2 receptor knockout mice.

Autor: Posa L; Neurobiological Psychiatry Unit, Department of Psychiatry, McGill University Health Center, McGill University, Montreal, QC, Canada.; Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada., Lopez-Canul M; Neurobiological Psychiatry Unit, Department of Psychiatry, McGill University Health Center, McGill University, Montreal, QC, Canada., Rullo L; Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna, Bologna, Italy., De Gregorio D; Neurobiological Psychiatry Unit, Department of Psychiatry, McGill University Health Center, McGill University, Montreal, QC, Canada., Dominguez-Lopez S; Neurobiological Psychiatry Unit, Department of Psychiatry, McGill University Health Center, McGill University, Montreal, QC, Canada., Kaba Aboud M; Neurobiological Psychiatry Unit, Department of Psychiatry, McGill University Health Center, McGill University, Montreal, QC, Canada., Caputi FF; Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna, Bologna, Italy., Candeletti S; Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna, Bologna, Italy., Romualdi P; Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna, Bologna, Italy., Gobbi G; Neurobiological Psychiatry Unit, Department of Psychiatry, McGill University Health Center, McGill University, Montreal, QC, Canada.; Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada.
Jazyk: angličtina
Zdroj: Journal of pineal research [J Pineal Res] 2020 Oct; Vol. 69 (3), pp. e12671. Date of Electronic Publication: 2020 Jun 08.
DOI: 10.1111/jpi.12671
Abstrakt: Melatonin, a neurohormone that binds to two G protein-coupled receptors MT 1 and MT 2, is involved in pain regulation, but the distinct role of each receptor has yet to be defined. We characterized the nociceptive responses of mice with genetic inactivation of melatonin MT 1 (MT 1 -/- ), or MT 2 (MT 2 -/- ), or both MT 1 /MT 2 (MT 1 -/- /MT 2 -/- ) receptors in the hot plate test (HPT), and the formalin test (FT). In HPT and FT, MT 1 -/- display no differences compared to their wild-type littermates (CTL), whereas both MT 2 -/- and MT 1 -/- /MT 2 -/- mice showed a reduced thermal sensitivity and a decreased tonic nocifensive behavior during phase 2 of the FT in the light phase. The MT 2 partial agonist UCM924 induced an antinociceptive effect in MT 1 -/- but not in MT 2 -/- and MT 1 -/- /MT 2 -/- mice. Also, the competitive opioid antagonist naloxone had no effects in CTL, whereas it induced a decrease of nociceptive thresholds in MT 2 -/- mice. Our results show that the genetic inactivation of melatonin MT 2 , but not MT 1 receptors, produces a distinct effect on nociceptive threshold, suggesting that the melatonin MT 2 receptor subtype is selectively involved in the regulation of pain responses.
(© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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