Tetraspanin CD53 Promotes Lymphocyte Recirculation by Stabilizing L-Selectin Surface Expression.

Autor:	Demaria MC; Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia., Yeung L; Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia; Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, 246 Clayton Road, Clayton, VIC 3168, Australia., Peeters R; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands., Wee JL; Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia; Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, 246 Clayton Road, Clayton, VIC 3168, Australia., Mihaljcic M; Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia., Jones EL; Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia., Nasa Z; Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia., Alderuccio F; Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia., Hall P; Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, 246 Clayton Road, Clayton, VIC 3168, Australia., Smith BC; Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, 246 Clayton Road, Clayton, VIC 3168, Australia., Binger KJ; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC 3086, Australia., Hammerling G; Molecular Immunology, German Cancer Research Center, Heidelberg, Germany., Kwok HF; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macau SAR, China., Newman A; Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK., Ager A; Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK., van Spriel A; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands., Hickey MJ; Centre for Inflammatory Diseases, Monash University Department of Medicine, Monash Medical Centre, 246 Clayton Road, Clayton, VIC 3168, Australia., Wright MD; Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia. Electronic address: mark.wright@monash.edu.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2020 May 22; Vol. 23 (5), pp. 101104. Date of Electronic Publication: 2020 Apr 27.
DOI:	10.1016/j.isci.2020.101104
Abstrakt:	Tetraspanins regulate key processes in immune cells; however, the function of the leukocyte-restricted tetraspanin CD53 is unknown. Here we show that CD53 is essential for lymphocyte recirculation. Lymph nodes of Cd53 -/- mice were smaller than those of wild-type mice due to a marked reduction in B cells and a 50% decrease in T cells. This reduced cellularity reflected an inability of Cd53 -/- B and T cells to efficiently home to lymph nodes, due to the near absence of L-selectin from Cd53 -/- B cells and reduced stability of L-selectin on Cd53 -/- T cells. Further analyses, including on human lymphocytes, showed that CD53 stabilizes L-selectin surface expression and may restrain L-selectin shedding via both ADAM17-dependent and ADAM17-independent mechanisms. The disruption in lymphocyte recirculation in Cd53 -/- mice led to impaired immune responses dependent on antigen delivery to lymph nodes. Together these findings demonstrate an essential role for CD53 in lymphocyte trafficking and immunity. Competing Interests: Declaration of Interests The authors declare no competing interests. (Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu