Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro .

Autor:	Carneiro-Goetten JOL; Programa de Pós-graduação em Ciências da Saúde, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil., Rodrigues BS; Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil., Nogoceke RA; Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil., do Nascimento TG; Programa de Pós-graduação em Ciências da Saúde, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil., Moreno-Amaral AN; Programa de Pós-graduação em Ciências da Saúde, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil., Stuelp-Campelo PM; Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil., Elifio-Esposito S; Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
Jazyk:	angličtina
Zdroj:	The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2020 May 11; Vol. 26, pp. e20190073. Date of Electronic Publication: 2020 May 11 (Print Publication: 2020).
DOI:	10.1590/1678-9199-JVATITD-2019-0073
Abstrakt:	Background: Neuroblastoma is a pediatric tumor with a mortality rate of 40% in the most aggressive cases. Tumor microenvironment components as immune cells contribute to the tumor progression; thereby, the modulation of immune cells to a pro-inflammatory and antitumoral profile could potentialize the immunotherapy, a suggested approach for high-risk patients. Preview studies showed the antitumoral potential of BJcuL, a C- type lectin isolated from Bothrops jararacussu venom. It was able to induce immunomodulatory responses, promoting the rolling and adhesion of leukocytes and the activation of neutrophils. Methods: SK-N-SH cells were incubated with conditioned media (CM) obtained during the treatment of neutrophils with BJcuL and fMLP, a bacteria-derived peptide highly effective for activating neutrophil functions. Then we evaluated the effect of the same stimulation on the co-cultivation of neutrophils and SK-N-SH cells. Tumor cells were tested for viability, migration, and invasion potential. Results: In the viability assay, only neutrophils treated with BJcuL (24 h) and cultivated with SK-N-SH were cytotoxic. Migration of tumor cells decreased when incubated directly (p < 0.001) or indirectly (p < 0.005) with untreated neutrophils. When invasion potential was evaluated, neutrophils incubated with BJcuL reduced the total number of colonies of SK-N-SH cells following co-cultivation for 24 h (p < 0.005). Treatment with CM resulted in decreased anchorage-free survival following 24 h of treatment (p < 0.001). Conclusion: Data demonstrated that SK-N-SH cells maintain their migratory potential in the face of neutrophil modulation by BJcuL, but their invasive capacity was significantly reduced. Competing Interests: Competing interests: The authors declare that they have no competing interests.
Databáze:	MEDLINE
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