| Autor: |
Ramos AGB; Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil., de Menezes IRA; Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil., da Silva MSA; Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil., Torres Pessoa R; Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil., de Lacerda Neto LJ; Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil., Rocha Santos Passos F; Department of Physiology, Federal University of Sergipe, São Cristóvão, Aracaju - SE 49100-000, Brazil., Melo Coutinho HD; Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, Brazil., Iriti M; Department of Agricultural and Environmental Sciences, Milan State University, via G. Celoria 2, 20133 Milan, Italy., Quintans-Júnior LJ; Department of Physiology, Federal University of Sergipe, São Cristóvão, Aracaju - SE 49100-000, Brazil. |
| Abstrakt: |
Isopulegol (ISO) is an alcoholic monoterpene widely found in different plant species, such as Melissa officinalis, and has already been reported to have a number of pharmacological properties. Like other terpenes, ISO is a highly volatile compound that is slightly soluble in water, so its inclusion into cyclodextrins (CDs) is an interesting approach to increase its solubility and bioavailability. Thus, our aim was to evaluate the antiedematogenic and anti-inflammatory activity of isopulegol and a β-cyclodextrin-isopulegol inclusion complex (ISO/β-CD) in rodent models. For the anti-inflammatory activity evaluation, antiedematogenic plethysmometry and acute (peritonitis and pleurisy), as well as chronic (cotton pellet-induced granuloma) anti-inflammatory models, were used. The docking procedure is used to evaluate, analyze, and predict their binding mode of interaction with H1 and Cox-2 receptors. The animals (n = 6) were divided into groups: ISO and ISO/β-CD, negative control (saline), and positive control (indomethacin and promethazine). ISO and ISO/β-CD were able to reduce acute inflammatory activity by decreasing albumin extravasation, leukocyte migration, and MPO concentration, and reducing exudate levels of IL-1β and TNF-α. ISO and ISO/β-CD significantly inhibited edematogenic activity in carrageenan- and dextran-induced paw edema. Moreover, both significantly reduced chronic inflammatory processes, given the lower weight and protein concentration of granulomas in the foreign body granulomatous inflammation model. The results suggest that the inclusion of ISO in β-cyclodextrins improves its pharmacological properties, with the histamine and prostaglandin pathways as probable mechanisms of inhibition, and also reinforces the anti-inflammatory profile of this terpene. |
